Photoaffinity labeling of peripheral benzodiazepine receptors in R-3327 dunning prostatic tumors

Photoaffinity labeling of peripheral benzodiazepine receptors (PBZr) in mitochondrial and microsomal fractions from AT-1, H, and G Dunning R-3327 tumor sublines was performed using the photoaffinity ligand 3H-PK 14105. Subsequent sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE...

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Bibliographic Details
Published in:Biochemical pharmacology 1996-04, Vol.51 (8), p.1009-1013
Main Authors: Alenfall, Jan, Batra, Satish
Format: Article
Language:English
Subjects:
Affinity Labels
Animals
benzodiazepine
Biological and medical sciences
Dunning tumors
Electrophoresis, Gel, Two-Dimensional
Flunitrazepam
Gynecology. Andrology. Obstetrics
Isoquinolines
Male
Male genital diseases
Medical sciences
mitochondria
neoplasm
photoaffinity labeling
PK 14105
Prostatic Neoplasms - metabolism
Proteins - analysis
Rats
Receptors, GABA-A - analysis
Subcellular Fractions - metabolism
Tumors
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Summary:Photoaffinity labeling of peripheral benzodiazepine receptors (PBZr) in mitochondrial and microsomal fractions from AT-1, H, and G Dunning R-3327 tumor sublines was performed using the photoaffinity ligand 3H-PK 14105. Subsequent sodium dodecyl sulphate (SDS)-polyacrylamide gel electrophoresis (PAGE) revealed specific labeling of a 17 kDa protein in the microsomal fractions from these tumors. In the mitochondrial fractions, however, two bands with the apparent molecular mass of 17 and 18 kDa in AT-1 and H tumors were identified, whereas only the 17 kDa band was present in G tumors. Using another ligand, 3H-flunitrazepam, to photoaffinity label PBZr, proteins with molecular masses of 17, 30, 42, and 48 kDa were identified. These data suggest the possibility of different subclasses of PBZrs in Dunning prostatic tumors.
ISSN:0006-2952
1873-2968
DOI:10.1016/0006-2952(95)02400-X