Hirudin stimulates prostacyclin but not endothelin-1 production in cultured human vascular endothelial cells

To study the effect of hirudin on endothelial cell prostacyclin (PGI 2) and endothelin-1 (ET-1) production, we cultured human umbilical vein endothelial cells (HUVECs), stimulated them with 0.00001–10 kU/l of hirudin for 12–24 hours, and measured by radioimmunoassays the concentrations of 6-ketopros...

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Bibliographic Details
Published in:Thrombosis research 1996-03, Vol.81 (6), p.635-640
Main Authors: Turunen, Pertti, Mikkola, Tomi, Ylikorkala, Olavi, Viinikka, Lasse
Format: Article
Language:English
Subjects:
Anticoagulants - pharmacology
Biological and medical sciences
Blood. Blood coagulation. Reticuloendothelial system
Cells, Cultured
Endothelin-1 - biosynthesis
Endothelium, Vascular - cytology
Endothelium, Vascular - drug effects
Epoprostenol - biosynthesis
Hirudins - pharmacology
Humans
Medical sciences
Pharmacology. Drug treatments
platelet aggregation
Stimulation, Chemical
thrombin
thrombosis
vasodilatation
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Summary:To study the effect of hirudin on endothelial cell prostacyclin (PGI 2) and endothelin-1 (ET-1) production, we cultured human umbilical vein endothelial cells (HUVECs), stimulated them with 0.00001–10 kU/l of hirudin for 12–24 hours, and measured by radioimmunoassays the concentrations of 6-ketoprostaglandinF 1 alfa (6-keto, a metabolite of PGI 2) and ET-1 in the incubation medium. In incubation medium containing 10% serum hirudin stimulated PGI 2-production dose-dependently. The lowest stimulatory hirudin concentration was 0.001 kU/l, which increased the concentration of 6-keto by 10.8±4.4% (mean±S.E) (p
ISSN:0049-3848
1879-2472
DOI:10.1016/0049-3848(96)00039-4