Genes encoding giant danio and golden shiner ependymin

Ependymin (EPN) is a brain glycoprotein that functions as a neurotrophic factor in optic nerve regeneration and long-term memory consolidation in goldfish. To date, true epn genes have been characterized in one order of teleost fish, Cypriniformes. In the study presented here, polymerase chain react...

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Bibliographic Details
Published in:Neurochemical research 1996-03, Vol.21 (3), p.377-384
Main Authors: ADAMS, D. S, KIYOKAWA, M, GETMAN, M. E, SHASHOUA, V. E
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biochemistry and metabolism
Biological and medical sciences
Brain - metabolism
Carps
Central nervous system
Cloning, Molecular
Conserved Sequence
Cypriniformes
Cypriniformes - genetics
DNA Primers
Freshwater
Fundamental and applied biological sciences. Psychology
Goldfish
Molecular Sequence Data
Nerve Regeneration
Nerve Tissue Proteins - biosynthesis
Nerve Tissue Proteins - chemistry
Nerve Tissue Proteins - genetics
Optic Nerve - physiology
Polymerase Chain Reaction
Protein Conformation
Recombinant Proteins - biosynthesis
Recombinant Proteins - chemistry
Sequence Homology, Amino Acid
Species Specificity
Vertebrates: nervous system and sense organs
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Summary:Ependymin (EPN) is a brain glycoprotein that functions as a neurotrophic factor in optic nerve regeneration and long-term memory consolidation in goldfish. To date, true epn genes have been characterized in one order of teleost fish, Cypriniformes. In the study presented here, polymerase chain reactions were used to analyze the complete epn genes, gd (1480 bp), and sh (2071 bp), from Cypriniformes giant danio and shiner, respectively. Southern hybridizations demonstrated the existence of one copy of each gene per corresponding haploid genome. Each gene was found to contain six exons and five introns. Gene gd encodes a predicted 218-amino acid (aa) protein GD 93 percent conserved to goldfish EPN, while sh encodes a predicted 214-aa protein SH 91 percent homologous to goldfish. Evidence is presented classifying proteins previously termed "EPNs" into two major categories: true EPNs and non-EPN cerebrospinal fluid glycoproteins. Proteins GD and SH contain all the hallmark, features of true EPNs.
ISSN:0364-3190
1573-6903
DOI:10.1007/BF02531655