Genomic instability in MycER-activated Rat1A-MycER cells

The deregulated expression of c-Myc protein is associated with the non-random locus-specific amplification of the dihydrofolate reductase (DHFR) gene. This study was performed to determine whether additional chromosomal aberrations occur when c-Myc protein levels are up-regulated for prolonged perio...

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Bibliographic Details
Published in:Chromosome research 1996-08, Vol.4 (5), p.365-371
Main Authors: Mai, S, Fluri, M, Siwarski, D, Huppi, K
Format: Article
Language:English
Subjects:
Animals
Base Sequence
Cell Line
Chromosome Aberrations
DNA Primers - genetics
Gene Expression Regulation
In Situ Hybridization, Fluorescence
Proto-Oncogene Proteins c-myc - genetics
Rats
Tetrahydrofolate Dehydrogenase - genetics
Up-Regulation
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Summary:The deregulated expression of c-Myc protein is associated with the non-random locus-specific amplification of the dihydrofolate reductase (DHFR) gene. This study was performed to determine whether additional chromosomal aberrations occur when c-Myc protein levels are up-regulated for prolonged periods. To this end, we have used Rat1A-MycER cells, which allow the experimental regulation of Myc protein levels. We examined the genomic stability of Rat1A-MycER cells cultivated in either the absence or the presence of estrogen, which reportedly activates the chimeric MycER protein in these cells. Following prolonged periods of MycER activation, Rat1A-Mycer cells exhibited irreversible chromosomal aberrations. The aberrations included numerical changes, chromosome breakage, the formation of circular chromosomal structures, chromosome fusions, and extrachromosomal elements.
ISSN:0967-3849
1573-6849
DOI:10.1007/BF02257272