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Production, characterization and control of a Neisseria meningitidis hexavalent class 1 outer membrane protein containing vesicle vaccine

An experimental serogroup B meningococcal vaccine was prepared from two genetically engineered strains; each expressing three different class 1 outer membrane proteins (OMPs) (PorA). The two strains expressed the subtypes P1.7,16;P1.5,2;P1.19,15 and P1.5 c,10;P1.12,13;P1.7 h,4, respectively. Outer m...

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Bibliographic Details
Published in:Vaccine 1996-07, Vol.14 (10), p.1001-1008
Main Authors: Claassen, Ivo, Meylis, Jolanda, van der Ley, Peter, Peeters, Carla, Brons, Hans, Robert, Jolanda, Borsboom, Dominique, van der Ark, Arno, van Straaten, Ineke, Roholl, Paul, Kuipers, Betsy, Poolman, Jan
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Language:English
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Summary:An experimental serogroup B meningococcal vaccine was prepared from two genetically engineered strains; each expressing three different class 1 outer membrane proteins (OMPs) (PorA). The two strains expressed the subtypes P1.7,16;P1.5,2;P1.19,15 and P1.5 c,10;P1.12,13;P1.7 h,4, respectively. Outer membrane vesicles (OMV) were prepared from these strains by deoxycholate extraction, mixed with aluminiumphosphate as adjuvant and formulated to final vaccines. The class 1 OMPs represent ca 90% of the protein in the vaccine. The vaccine was found safe for human use and induced a bactericidal immune response in mice against five of the six wild type strains, which served as donors for the various porA genes.
ISSN:0264-410X
1873-2518
DOI:10.1016/0264-410X(96)00020-5