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Growth of Chlamydia pneumoniae in cultured human peripheral blood mononuclear cells and induction of a cytokine response

In vitro infection of freshly isolated human peripheral blood mononuclear cells (HPBMC) with Chlamydia pneumoniae was found to induce a production of tumour necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and interferon alpha (IFN-alpha). The secretion was dep...

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Bibliographic Details
Published in:Microbial pathogenesis 1996-09, Vol.21 (3), p.215-221
Main Authors: KAUKORANTA-TOLVANEN, S.-S. E, TEPPO, A.-M, LAITINEN, K, SAIKKU, P, LINNAVUORI, K, LEINONEN, M
Format: Article
Language:English
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Summary:In vitro infection of freshly isolated human peripheral blood mononuclear cells (HPBMC) with Chlamydia pneumoniae was found to induce a production of tumour necrosis factor alpha (TNF-alpha), interleukin-1 beta (IL-1 beta), interleukin-6 (IL-6) and interferon alpha (IFN-alpha). The secretion was dependent on the amount of infecting chlamydiae and most of it occurred during the first 12 to 24 h. Lipopolysaccharide (LPS) of Salmonella minnesota Rechemotype, used as a positive control for HPBMC activation, induced a release of TNF-alpha, IL-1 beta and IL-6, but not of IFN-alpha, similar to the effect of C. pneumoniae. Viable chlamydiae could not be recovered from HPBMCs infected immediately after their isolation, whereas HPBMCs which were cultured in vitro for 3 to 9 days before infection were able to maintain the growth of C. pneumoniae. Growth inside HPBMCs as well as induction of cytokine response may have a role in the pathogenesis of C. pneumoniae infection.
ISSN:0882-4010
1096-1208
DOI:10.1006/mpat.1996.0056