Regulation of Acetyl Coenzyme-A Carboxylase Gene in a Transgenic Animal Model

The gene for acetyl-CoA carboxylase, the rate-limiting enzyme in the biosynthesis of long-chain fatty acids, contains two promoters; promoter I (PI) and promoter II (PII) which are responsible for generation of class I and class II ACC mRNAs, respectively. Class I ACC mRNAs are present in adipose ti...

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Bibliographic Details
Published in:Biochemical and biophysical research communications 1996-10, Vol.227 (2), p.547-552
Main Authors: Ekhterae, Daryoush, Tae, Hyi-Jeong, Daniel, Samira, Moller, David E., Kim, Ki-Han
Format: Article
Language:English
Subjects:
Acetyl-CoA Carboxylase - biosynthesis
Acetyl-CoA Carboxylase - genetics
Adipose Tissue - enzymology
Animals
Chloramphenicol O-Acetyltransferase - biosynthesis
Female
Gene Expression Regulation, Enzymologic
Genes, Reporter
Liver - enzymology
Male
Mice
Mice, Inbred Strains
Mice, Transgenic
Organ Specificity
Promoter Regions, Genetic
Pseudopregnancy
Restriction Mapping
RNA, Messenger - biosynthesis
Transcription, Genetic
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Summary:The gene for acetyl-CoA carboxylase, the rate-limiting enzyme in the biosynthesis of long-chain fatty acids, contains two promoters; promoter I (PI) and promoter II (PII) which are responsible for generation of class I and class II ACC mRNAs, respectively. Class I ACC mRNAs are present in adipose tissue, but only a trace was found in the liver under normal physiological conditions. However, class I mRNAs were induced under stimulated lipogenic conditions. To investigate how PI is regulatedin vivo,we generated transgenic mice containing a reporter gene under the control of PI. In transgenic mice, PI is generally inactive and a small amount of PI activity was found only in the adipose tissues of female animals. Stimulated lipogenic conditions activated PI about 17-fold over normal conditions and again only in white adipose tissues of female animals.
ISSN:0006-291X
1090-2104
DOI:10.1006/bbrc.1996.1543