Cloning and chromosomal location of human genes inducible by type I interferon

When cells are treated with interferon several new proteins are induced. We have isolated by differential screening two cDNA clones corresponding to human genes inducible by IFN-alpha, termed IFI-4 and IFI-54K. The accumulation of the corresponding mRNA was followed as a function of either IFN dose...

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Bibliographic Details
Published in:Somatic cell and molecular genetics 1988-09, Vol.14 (5), p.415-426
Main Authors: Wathelet, M G, Szpirer, J, Nols, C B, Clauss, I M, De Wit, L, Islam, M Q, Levan, G, Horisberger, M A, Content, J, Szpirer, C
Format: Article
Language:English
Subjects:
2',5'-Oligoadenylate Synthetase - genetics
Animals
Blotting, Northern
Blotting, Southern
Cell Line
Chromosome Mapping
Chromosomes, Human, Pair 1
Chromosomes, Human, Pair 10
Chromosomes, Human, Pair 12
Chromosomes, Human, Pair 13
Cloning, Molecular
DNA - genetics
DNA Probes
Gene Expression Regulation
Genes
Humans
Hybrid Cells
Interferon Type I - physiology
Molecular Weight
Pseudogenes
Restriction Mapping
RNA Probes
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Summary:When cells are treated with interferon several new proteins are induced. We have isolated by differential screening two cDNA clones corresponding to human genes inducible by IFN-alpha, termed IFI-4 and IFI-54K. The accumulation of the corresponding mRNA was followed as a function of either IFN dose or of time. The IFI-4 and IFI-54K genes, as well as two previously isolated IFN-inducible genes, namely the IFI-56K and low-molecular-weight 2-5A synthetase, were localized on the human chromosomes. Using cloned probes on Southern blots of DNA from a panel of rodent-human somatic cell hybrids, we have assigned the IFI-4 gene to chromosome 1 and the gene coding for the low-molecular-weight 2-5A synthetase to chromosome 12. We also showed that the IFI-54K and IFI-56K genes, unlike most of the IFN-inducible genes, are syntenic. They are both located on chromosome 10. In addition, evidence is given for the presence of a pseudogene homologous to IFI-56K on chromosome 13.
ISSN:0740-7750
1572-9931
DOI:10.1007/BF01534709