Cross-reactivity of Shigella flexneri serotype 2a O antigen antibodies following immunization or infection

• Published in: Vaccine 1996-08, Vol.14 (11), p.1062-1068
• Main Authors: Van De Verg, Lillian L., Bendiuk, Natalie O., Kotloff, Karen, Marsh, Michelle M., Ruckert, Jennifer L., Puryear, Jill L., Taylor, David N., Hartman, Antoinette B.
• Format: Article
• Language: English
• Subjects: Adult; Animals; antibodies; Antibodies, Bacterial - blood; Antibodies, Bacterial - immunology; Bacterial Vaccines - immunology; Bacteriology; Biological and medical sciences; Carbohydrate Sequence; Cross Reactions; cross-reactivity; Dysentery, Bacillary - immunology; Enzyme-Linked Immunosorbent Assay; Fundamental and applied biological sciences. Psychology; Guinea Pigs; Haplorhini; Humans; Immunoglobulin A - blood; Immunoglobulin A - immunology; Immunoglobulin G - blood; Immunoglobulin G - immunology; Lipopolysaccharides - immunology; LPS; Microbiology; Molecular Sequence Data; O Antigens - immunology; Shigella flexneri; Shigella flexneri - classification; Shigella flexneri - immunology; Shigella flexneri 2a; vaccine; Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies; Vaccines, Synthetic - immunology
• ISSN: 0264-410X; 1873-2518
• DOI: 10.1016/0264-410X(96)00006-0

To study the cross-reactivity pattern of Shigella flexneri 2a O-antigen antibodies, sera from humans and monkeys challenged with S. flexneri 2a, and from humans and guinea pigs immunized with a recombinant vaccine expressing serotype 2a O-antigen, were tested against a panel of lipopolysaccharide extracted from heterologous S. flexneri. Sera from the two groups of humans, who were volunteers in either a clinical challenge or vaccination study, showed similar patterns: cross-reactivity was more often seen with IgA antibodies, and these were mostly cross-reactive with serotype 2b, which shares the type II antigen, and serotypes 1a, 5a, and Y, which share 4 or 3,4 group antigen, with 2a. The majority of sera from immunized guinea pigs showed both IgG and IgA cross-reactivity with 1a, 5a, and Y, but not 2b. The majority of sera from challenged monkeys showed cross-reactivity with almost all flexneri serotypes tested, with 1a, 2b, and Y being recognized most often, and the cross-reactive antibodies were more often IgG than IgA. These results show that either immunization or challenge with the 2a serotype induces cross-reactive antibodies which recognize similar subsets of heterologous serotypes, and suggest that it may be possible to design multivalent vaccines against S. flexneri.