Use of ELISA for in vitro potency test of rabies vaccines for animal use

The use of ELISA for the potency test of inactivated rabies vaccines for animal use was evaluated. Sandwich ELISA was developed, using monoclonal antibodies which enabled to recognize the glycoprotein (G-protein) of rabies virus and showed a protective effect in mice. Soluble G-protein which display...

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Bibliographic Details
Published in:Biologicals 1996-06, Vol.24 (2), p.95-101
Main Authors: GAMOH, K, SENDA, M, ITOH, O, MURAMATSU, M, HIRAYAMA, N, KOIKE, R, ENDOH, Y. S, MINAMOTO, N
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Blotting, Western
Chromatography, High Pressure Liquid
Enzyme-Linked Immunosorbent Assay - methods
Fundamental and applied biological sciences. Psychology
Guinea Pigs
Mice
Microbiology
Rabies Vaccines
rabies virus
Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies
Virology
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Summary:The use of ELISA for the potency test of inactivated rabies vaccines for animal use was evaluated. Sandwich ELISA was developed, using monoclonal antibodies which enabled to recognize the glycoprotein (G-protein) of rabies virus and showed a protective effect in mice. Soluble G-protein which displays an ELISA activity but a low immunogenicity was removed by gel filtration before ELISA was carried out. The ELISA titres of the first fraction of vaccines separated by gel filtration were correlated with the neutralizing antibody titres of vaccinated guinea-pigs. Our results suggest that the ELISA titres of the first fraction of vaccines separated by gel filtration reflected the protective G-protein contents for the in vitro potency test of rabies vaccines compared with the current in vivo test.
ISSN:1045-1056
1095-8320
DOI:10.1006/biol.1996.0012