Effects of LHRH, FSH and Activin A on Follistatin Secretion from Cultured Rat Anterior Pituitary Cells

We demonstrated the release of follistatin, an activin-binding protein, from cultured rat anterior pituitary cells by measuring immunoreactive (ir-) follistatin in a specific immunoradiometric assay. Ir-follistatin release gradually increased in cultures over 1-18 days and reached its maximal level...

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Published in:ENDOCRINE JOURNAL 1996, Vol.43(3), pp.321-327
Main Authors: LIU, ZHONC-HUI, SHINTANI, YASUMI, SAKAMOTO, YUKIHIRO, HARADA, KAZUYO, ZHANG, CHEN-YU, FUJINAKA, YUICHI, ABE, MASAHIRO, GOTO, TETSUYA, SAITO, SHIRO
Format: Article
Language:English
Subjects:
Activin
Activins
Animals
Anterior pituitary cells
Blotting, Western
Cells, Cultured
Female
Follicle Stimulating Hormone - pharmacology
Follistatin
FSH
Glycoproteins - analysis
Glycoproteins - drug effects
Glycoproteins - metabolism
Gonadotropin-Releasing Hormone - pharmacology
Growth Substances - pharmacology
Humans
Immunoradiometric Assay
Immunoradiometric assay (IRMA)
Inhibins - pharmacology
LHRH
Male
Pituitary Gland, Anterior - cytology
Pituitary Gland, Anterior - drug effects
Pituitary Gland, Anterior - metabolism
Rabbits
Random Allocation
Rats
Rats, Wistar
Time Factors
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Summary:We demonstrated the release of follistatin, an activin-binding protein, from cultured rat anterior pituitary cells by measuring immunoreactive (ir-) follistatin in a specific immunoradiometric assay. Ir-follistatin release gradually increased in cultures over 1-18 days and reached its maximal level at 12-15 days of incubation. The basal ir-follistatin levels in the culture media increased about 3- (P< 0.01) and 5-fold (P
ISSN:0918-8959
1348-4540
DOI:10.1507/endocrj.43.321