Widespread immunoreactivity of presenilin in neurons of normal and Alzheimer's disease brains : double-labeling immunohistochemical study

The immunolocalization of presenilin in human brain was studied using two antibodies raised against different portions of presenilin 1 (S182) protein. A granular staining was found in the cytoplasm of neurons in cortical layers III and V. One of the antibodies, also reactive to presenilin 2 (E5-1) p...

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Bibliographic Details
Published in:Acta neuropathologica 1996-10, Vol.92 (4), p.325-330
Main Authors: UCHIHARA, T, EL HACHIMI, H. K, DUYCKAERTS, C, FONCIN, J.-F, FRASER, P. E, LEVESQUE, L, GEORGE-HYSLOP, P. H. S, HAUW, J.-J
Format: Article
Language:English
Subjects:
Aged
Aged, 80 and over
Alzheimer Disease - immunology
Alzheimer Disease - metabolism
Alzheimer Disease - pathology
Antibodies, Monoclonal - chemistry
Antigen-Antibody Reactions
Biological and medical sciences
Brain Chemistry - immunology
Degenerative and inherited degenerative diseases of the nervous system. Leukodystrophies. Prion diseases
Humans
Immunohistochemistry
Medical sciences
Membrane Proteins - immunology
Neurology
Neurons - immunology
Neurons - pathology
Presenilin-1
Presenilin-2
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Summary:The immunolocalization of presenilin in human brain was studied using two antibodies raised against different portions of presenilin 1 (S182) protein. A granular staining was found in the cytoplasm of neurons in cortical layers III and V. One of the antibodies, also reactive to presenilin 2 (E5-1) protein, additionally stained dendrites and axons. This was seen in normal brains as well as in brains affected by Alzheimer's disease. Less prominent immunolabeling was noted in some senile plaques. No relationship to neurofibrillary tangles was found in double-labeling experiments combined with anti-paired helical filament-tau antibody (AT8). The widespread expression of presenilin in normal brain suggests a physiological role of the protein.
ISSN:0001-6322
1432-0533
DOI:10.1007/s004010050526