Chemotactic Activity of Recombinant Human Granulocyte Colony-Stimulating Factor

• Published in: Blood 1988-11, Vol.72 (5), p.1456-1460
• Main Authors: Wang, Ji Ming, Chen, Zhen Guo, Colella, Silvia, Bonilla, Mary Ann, Welte, Karl, Bordignon, Claudio, Mantovani, Alberto
• Format: Article
• Language: English
• Subjects: Antibodies, Monoclonal - immunology; Biological and medical sciences; Cell physiology; Chemotaxis, Leukocyte - drug effects; Collodion; Colony-Stimulating Factors - pharmacology; Endothelium - physiology; Filtration - instrumentation; Fundamental and applied biological sciences. Psychology; Granulocyte Colony-Stimulating Factor; Granulocyte-Macrophage Colony-Stimulating Factor; Growth Substances - pharmacology; Humans; In Vitro Techniques; Lymphocytes - immunology; Molecular and cellular biology; Monocytes - physiology; Motility and taxis; Neutrophils - physiology; Polycarboxylate Cement; Recombinant Proteins - pharmacology
• ISSN: 0006-4971; 1528-0020
• DOI: 10.1182/blood.V72.5.1456.1456

Recombinant human granulocyte colony-stimulating factor (rhG-CSF) induced migration across polycarbonate filters of human polymorphonuclear leukocytes (PMN). rhG-CSF was active in inducing PMN migration at concentrations ≥10 to 100 U/mL (7 to 70 ng/mL). rhG-CSF did not contain appreciable levels of endotoxin contamination as assessed by Limulus amebocyte assay, and Polymixin B did not affect the chemotactic activity of rhG-CSF. A monoclonal anti-G-CSF antibody blocked the induction of migration by G-CSF, thus establishing that the cytokine was responsible for the activity of the recombinant preparation. Checkerboard analysis was performed by seeding different concentrations of G-CSF above and/or below the filter and revealed that the migratory response to this cytokine was best observed in the presence of a positive concentration gradient between the lower and upper compartments of the chamber, thus indicating an actual chemotactic effect. When different migrating cells were examined, rhG-CSF was inactive on large granular lymphocytes and endothelial cells under conditions in which appropriate reference attractants were active. In contrast, rhG-CSF elicited a chemotactic response in monocytes inhibited by specific antibody. Thus, G-CSF is a chemotactic signal for phagocytes. This cytokine, when produced at inflammatory sites, may contribute to the recruitment of phagocytes from the blood compartment to amplify resistance against certain noxious agents.