Assignment of genes coding for leukocyte surface molecules to river buffalo chromosomes

• Published in: Veterinary immunology and immunopathology 1996-08, Vol.52 (4), p.435-443
• Main Authors: El Nahas, S.M., Ramadan, H.A., Abou-Mossallem, A.A., Kurucz, E., Vilmos, P., Ando, I.
• Format: Article
• Language: English
• Subjects: Animals; Antibodies, Monoclonal - immunology; ANTICORPS MONOCLONAL; ANTICUERPOS MONOCLONALES; ANTIGENE; ANTIGENOS; ANTIGENS; Blotting, Western; BUFALO DE AGUA; Buffaloes; BUFFLE D'EAU; CHROMOSOME; Chromosome Mapping; CHROMOSOMES; Cricetinae; Cricetulus; CROMOSOMAS; Fluorescent Antibody Technique, Indirect; GENE LOCATION; Gene map; GENETIC MARKERS; HAMSTER; HAMSTERS; HIBRIDOS SOMATICOS; Histocompatibility Antigens Class I - genetics; Histocompatibility Antigens Class I - immunology; Histocompatibility Antigens Class II - genetics; Histocompatibility Antigens Class II - immunology; Hybrid Cells; HYBRIDE SOMATIQUE; LEUCOCITOS; LEUCOCYTE; Leukocyte antigens; LEUKOCYTES; LOCALISATION DE GENE; LOCALIZACION DE GENES; Major Histocompatibility Complex - genetics; Major Histocompatibility Complex - immunology; MARCADORES GENETICOS; MARQUEUR GENETIQUE; MONOCLONAL ANTIBODIES; River buffalo; SOMATIC HYBRIDS; Synteny; WATER BUFFALOES
• ISSN: 0165-2427; 1873-2534
• DOI: 10.1016/0165-2427(96)05597-3

A panel of 35 somatic cell hybrids between river buffalo ( Bubalus bubalis L.) and Chinese hamster was used in combination with 40 monoclonal antibodies to find synteny among leukocyte antigens and between already established synteny groups and leukocyte antigens. Six synteny groups were identified and three sets of markers were related to already existing synteny groups. Antibody IL-A165 detected CD71, which is syntenic with synteny groups U10 and U25, confirming that U10 and U25 are both are on a biarmed river buffalo chromosome. This would imply that U25 could be assigned to the short arm of the river buffalo chromosome 1 (BBU 1p), as U10 has previously been assigned to this chromosome. Antibodies Buf 32, Buf 24 and BAGB27 detected an antigen syntenic with U1 and U7. This finding suggests that U1, which is assigned to cattle chromosome 16, which corresponds to river buffalo chromosome 5q, is syntenic with U7, and that U7 is located on the short arm of buffalo chromosome 5. Furthermore, the gene coding for CD14 is assigned to U16, which is on cattle chromosome 11 and expected to be on river buffalo chromosome 12.