Spectrophotometric determination of hydrogen peroxide: catalase activity and rates of hydrogen peroxide removal by erythrocytes

A new method of hydrogen peroxide determination for the measurement of catalase activity and rates of hydrogen peroxide removal by erythrocytes was described. Hydrogen peroxide was determined by converting it to the indamine dye with a water-soluble ironporphyrin and measuring the absorbance at 590...

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Bibliographic Details
Published in:Clinica chimica acta 1996-10, Vol.254 (2), p.101-112
Main Authors: Masuoka, Noriyoshi, Wakimoto, Masahiro, Ubuka, Toshihiko, Nakano, Taku
Format: Article
Language:English
Subjects:
Acatalasemic erythrocytes
Amitrole - pharmacology
Animals
Biological and medical sciences
Catalase - metabolism
Catalase activity
Erythrocytes - drug effects
Erythrocytes - enzymology
Erythrocytes - metabolism
Humans
Hydrogen Peroxide - metabolism
Hydrogen peroxide determination
Hydrogen peroxide removal by erythrocytes
Investigative techniques, diagnostic techniques (general aspects)
Medical sciences
Mice
Miscellaneous. Technology
Pathology. Cytology. Biochemistry. Spectrometry. Miscellaneous investigative techniques
Rats
Spectrum Analysis
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Summary:A new method of hydrogen peroxide determination for the measurement of catalase activity and rates of hydrogen peroxide removal by erythrocytes was described. Hydrogen peroxide was determined by converting it to the indamine dye with a water-soluble ironporphyrin and measuring the absorbance at 590 nm. This method was applied to the assay of catalase in hemolysates from human, rat and mouse blood. The activities obtained were in agreement with those obtained by other methods including UV method. The present method was also applied to the determination of rates of hydrogen peroxide removal by intact erythrocytes from human subjects, rats and mice. Data suggested that normal erythrocytes have substantial capacity to remove extracellular hydrogen peroxide. From the measurement of catalase activity in erythrocytes treated with 3-amino-1,2,4-triazole and rates of hydrogen peroxide removal by the erythrocytes, it is deduced that rate constants related to the hemoglobin content (k/g Hb) for hydrogen peroxide removal by catalase in normal and acatalasemic erythrocytes are 42.0 ± 6.0 and 8.0 ± 3.0, respectively.
ISSN:0009-8981
1873-3492
DOI:10.1016/0009-8981(96)06374-7