Putrescine Uptake by Alveolar Epithelial Cell Monolayers Exhibiting Differing Transepithelial Electrical Resistances

Rat alveolar type II cells were isolated following elastase digestion and cultured on polycarbonate filters at various densities and in different media. Two days after seeding, the cells formed a monolayer on the filters which consisted predominately of type II cells, these then de-differentiated to...

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Bibliographic Details
Published in:Journal of pharmaceutical sciences 1996-10, Vol.85 (10), p.1112-1116
Main Authors: Dickinson, Paul A., Evans, Jonathan P., Farr, Stephen J., Kellaway, Ian W., Appelqvist, Terence P., Hann, Anthony C., Richards, Roy J.
Format: Article
Language:English
Subjects:
Biological and medical sciences
Biological Transport
Cell Count
Cell Membrane Permeability
Cells, Cultured - chemistry
Cells, Cultured - metabolism
Culture Media
Electric Impedance
Epithelium - metabolism
General pharmacology
Mannitol - metabolism
Medical sciences
Pharmacokinetics. Pharmacogenetics. Drug-receptor interactions
Pharmacology. Drug treatments
Pulmonary Alveoli - chemistry
Pulmonary Alveoli - metabolism
Pulmonary Alveoli - ultrastructure
Putrescine - metabolism
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Summary:Rat alveolar type II cells were isolated following elastase digestion and cultured on polycarbonate filters at various densities and in different media. Two days after seeding, the cells formed a monolayer on the filters which consisted predominately of type II cells, these then de-differentiated to a alveolar type I-like cell monolayer by day 6. The seeding density and media utilized affected the transepithelial electrical resistance (TEER) generated by the monolayer. Only certain culture conditions allowed the production of a monolayer that mimics, putatively, the in vivo alveolar epithelium (TEER greater than 1000 Ω cm2). Vmax and Km values for the uptake of putrescine by monolayers exhibiting low and high TEERs on day 6 were determined. The capacity of the putrescine uptake mechanisms was greater in cell monolayers exhibiting a high TEER than those exhibiting a low TEER, suggesting that the TEER does not only measure the “tightness” of the monolayer but contains an element representative of the viability of the cell monolayer. The selection of appropriate TEERs for cell culture investigations is discussed.
ISSN:0022-3549
1520-6017
DOI:10.1021/js9504898