Identification and sequencing of a novel insertion sequence, IS1471, in Burkholderia cepacia strain 2a

A novel insertion sequence (IS), IS1471, has been identified which is inserted into IS element IS1071 possessed by plasmid pIJB1 in Burkholderia cepacia strain 2a. Nucleotide sequencing has revealed that IS1471 is 1112 bp in length and is flanked by 22/21-bp imperfect inverted repeats with a 3-bp du...

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Bibliographic Details
Published in:FEMS microbiology letters 1996-11, Vol.144 (2/3), p.203-206
Main Authors: Xia, X.S, Smith, A.R.W, Bruce, I.J
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Amino acids
Bacteriology
Base Sequence
Biological and medical sciences
Biological Sciences
Burkholderia cepacia
Burkholderia cepacia - genetics
DNA Transposable Elements - genetics
DNA, Bacterial - genetics
Fundamental and applied biological sciences. Psychology
genbank/u67938
Genes, Bacterial
Genetics
Homology
Insertion
Insertion sequence
IS1471
Microbiology
Molecular Sequence Data
Nucleotide sequence
Nucleotides
Open Reading Frames
Polymerase Chain Reaction
Polypeptides
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Summary:A novel insertion sequence (IS), IS1471, has been identified which is inserted into IS element IS1071 possessed by plasmid pIJB1 in Burkholderia cepacia strain 2a. Nucleotide sequencing has revealed that IS1471 is 1112 bp in length and is flanked by 22/21-bp imperfect inverted repeats with a 3-bp duplication of the target sequence. IS1471 contains a single open reading frame encoding a putative polypeptide of 345 amino acids with molecular mass of 39406 Da. Searches of DNA and protein sequence databases did not result in the detection of any homologous IS elements, suggesting that IS1471 is novel and may not belong to any known IS groups.
ISSN:0378-1097
1574-6968
DOI:10.1111/j.1574-6968.1996.tb08531.x