Anti-Glomerular Basement Membrane Disease: Role of Enzyme-Linked Immunosorbent Assays in Diagnosis

We compared two enzyme-linked immunosorbent assay (ELISA) tests with indirect immunofluorescence (IFA) for the measurement of anti-glomerular basement membrane (GBM) antibodies to study their sensitivity and specificity. The first ELISA test kit compared well to IFA (96.4% agreement, 93.3% sensitivi...

Full description

Saved in:
Bibliographic Details
Published in:Biochemical and molecular medicine 1996-10, Vol.59 (1), p.52-56
Main Authors: Litwin, Christine M., Mouritsen, C.Lars, Wilfahrt, Pamela A., Schroder, M.Carl, Hill, Harry R.
Format: Article
Language:English
Subjects:
Adolescent
Adult
Aged
Aged, 80 and over
Anti-Glomerular Basement Membrane Disease - diagnosis
Antibodies - metabolism
Basement Membrane - immunology
Child
Enzyme-Linked Immunosorbent Assay
Fluorescent Antibody Technique, Indirect
Humans
Middle Aged
Citations: Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:We compared two enzyme-linked immunosorbent assay (ELISA) tests with indirect immunofluorescence (IFA) for the measurement of anti-glomerular basement membrane (GBM) antibodies to study their sensitivity and specificity. The first ELISA test kit compared well to IFA (96.4% agreement, 93.3% sensitivity, 97.0% specificity). The second ELISA kit was much less sensitive (87.8% agreement, 63.3% sensitivity, 92.2% specificity). ELISA tests for the measurement of anti-GBM antibodies allow for quantitation and may have greater sensitivity than IFA. Sensitivity, however, may depend on the commercial test used.
ISSN:1077-3150
1095-5577
DOI:10.1006/bmme.1996.0064