Morphological characterization of bovine primordial follicles and their environment in vivo

Classically, primordial follicles have flattened pre-granulosa cells that become cuboidal and divide during follicular activation. This change in shape is thus an index of activation. To examine this and follicular morphology in cattle, ovaries were processed for light and electron microscopy (n = 2...

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Bibliographic Details
Published in:Biology of reproduction 1996-11, Vol.55 (5), p.1003-1011
Main Authors: Wezel, I.L. van (Flinders University of South Australia, Bedford Park, South Australia, Australia.), Rodgers, R.J
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cattle
Cell Nucleus - ultrastructure
CELLULE
CELULAS
CHIMIE
Cytoplasm - ultrastructure
DIMENSION
ESTRUCTURA CELULAR
Female
Fundamental and applied biological sciences. Psychology
Granulosa Cells - ultrastructure
IMMUNOLOGIE
INMUNOLOGIA
Mammalian female genital system
Microscopy, Electron
Morphology. Physiology
Oocytes - ultrastructure
OVAIRE
Ovarian Follicle - ultrastructure
OVARIOS
Ovary - blood supply
Ovary - cytology
QUIMICA
Stem Cells - ultrastructure
STRUCTURE CELLULAIRE
ULTRAESTRUCTURA
ULTRASTRUCTURE
VACA
VACHE
Vertebrates: reproduction
von Willebrand Factor - analysis
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Summary:Classically, primordial follicles have flattened pre-granulosa cells that become cuboidal and divide during follicular activation. This change in shape is thus an index of activation. To examine this and follicular morphology in cattle, ovaries were processed for light and electron microscopy (n = 21). In single sections (5 micromolar thick), primordial follicles had a mean ratio of maximum to minimum diameters of 1.33 +/- 0.18 (mean +/- SD; n = 317, randomly selected), indicating that they had a prolate rather than a spherical shape. The prolate shape was attributable to clustering of granulosa cells at two opposite poles on the long axis of each follicle; and in each histological section, 82.5% of primordial follicles (n = 317) had at least one cuboidal granulosa cell. More accurate measurements of primordial follicles indicated that they had three dimensions measured as length (45.4 +/- 2.4 microgram), breadth (26.8 +/- 1.5 micrometer), and depth ( 30.4 +/- 1.4 micrometer) (mean +/- SEM; n
ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod55.5.1003