An improved method to distinguish micrococcal nuclease sensitivity of chromatin

The resolution of the standard micrococcal nuclease assay for sensitivity of active chromatin has been enhanced by the inclusion of an additional step of digesting nuclease-digested DNA with a suitable restriction enzyme prior to Southern hybridization. The improved assay has been used to analyze th...

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Bibliographic Details
Published in:Journal of biochemical and biophysical methods 1996-10, Vol.33 (1), p.59-64
Main Authors: Hamid, Q.Anwar, Thanumalayan, Subramonian, Parnaik, Veena K
Format: Article
Language:English
Subjects:
Active chromatin
Animals
Blotting, Southern
Cell Differentiation - physiology
Chromatin - metabolism
Deoxyribonuclease I - metabolism
DNA - isolation & purification
DNA Probes
Lamin A
Lamin Type A
Lamins
Liver - chemistry
Liver - enzymology
Liver - growth & development
Micrococcal nuclease
Micrococcal Nuclease - metabolism
Nuclear lamina
Nuclear Proteins - metabolism
Rats
Rats, Wistar
Sensitivity and Specificity
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Summary:The resolution of the standard micrococcal nuclease assay for sensitivity of active chromatin has been enhanced by the inclusion of an additional step of digesting nuclease-digested DNA with a suitable restriction enzyme prior to Southern hybridization. The improved assay has been used to analyze the chromatin structure of the lamin A, albumin and α-fetoprotein genes during rat liver development.
ISSN:0165-022X
1872-857X
DOI:10.1016/0165-022X(96)00014-0