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(s 4dU) 35: a novel, highly potent oligonucleotide inhibitor of the human immunodeficiency virus type 1 reverse transcriptase
Oligodeoxycytidylates were converted to s 4dUMP-containing oligomers by treatment with liquid H 2S. The inhibitory potency of the modified oligonucleotides on human immunodeficiency virus type 1 reverse transcriptase depended on the chain length and on the percentage of modification. The most potent...
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| Published in: | FEBS letters 1996-10, Vol.396 (1), p.43-46 |
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| Main Authors: | , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c3350-5c37f4c3ae2b5d651ef250994bdf3c482d39f24a41e177ffd3a4bc9bd2a13bc63 |
| container_end_page | 46 |
| container_issue | 1 |
| container_start_page | 43 |
| container_title | FEBS letters |
| container_volume | 396 |
| creator | Tökés, Szilvia Aradi, Janos |
| description | Oligodeoxycytidylates were converted to s
4dUMP-containing oligomers by treatment with liquid H
2S. The inhibitory potency of the modified oligonucleotides on human immunodeficiency virus type 1 reverse transcriptase depended on the chain length and on the percentage of modification. The most potent reverse transcriptase inhibitor was (s
4dU)
35. The inhibitory pattern was competitive, when either poly(A)·(dT)
16 or poly(C)·(dG)
16 was used as template-primer (variable substrate), suggesting that the free enzyme interacts with (s
4dU)
35. The
K
i
values were 3.0 and 2.2 nM in the presence of poly(A)·(dT)
16 and poly(C)·(dG)
16, respectively. |
| doi_str_mv | 10.1016/0014-5793(96)01032-0 |
| format | article |
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4dUMP-containing oligomers by treatment with liquid H
2S. The inhibitory potency of the modified oligonucleotides on human immunodeficiency virus type 1 reverse transcriptase depended on the chain length and on the percentage of modification. The most potent reverse transcriptase inhibitor was (s
4dU)
35. The inhibitory pattern was competitive, when either poly(A)·(dT)
16 or poly(C)·(dG)
16 was used as template-primer (variable substrate), suggesting that the free enzyme interacts with (s
4dU)
35. The
K
i
values were 3.0 and 2.2 nM in the presence of poly(A)·(dT)
16 and poly(C)·(dG)
16, respectively.</description><identifier>ISSN: 0014-5793</identifier><identifier>EISSN: 1873-3468</identifier><identifier>DOI: 10.1016/0014-5793(96)01032-0</identifier><identifier>PMID: 8906863</identifier><language>eng</language><publisher>England: Elsevier B.V</publisher><subject>AIDS, acquired immunodeficiency syndrome ; AIDS/HIV ; Antitemplate ; Chromatography, High Pressure Liquid ; Deoxyuracil Nucleotides - chemistry ; DNA Primers ; HIV Reverse Transcriptase - antagonists & inhibitors ; HIV, human immunodeficiency virus ; Human immunodeficiency virus ; Inhibition ; Na-PPi, Na-pyrophosphate ; Oligo 4-thio-2′-deoxyuridylate ; Oligonucleotides - chemistry ; Oligonucleotides - pharmacology ; Reverse transcriptase ; Reverse Transcriptase Inhibitors - chemistry ; Reverse Transcriptase Inhibitors - pharmacology ; RT, reverse transcriptase ; Structure-Activity Relationship ; Sulfur - chemistry ; TCA, trichloroacetic acid</subject><ispartof>FEBS letters, 1996-10, Vol.396 (1), p.43-46</ispartof><rights>1996</rights><rights>FEBS Letters 396 (1996) 1873-3468 © 2015 Federation of European Biochemical Societies</rights><lds50>peer_reviewed</lds50><oa>free_for_read</oa><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c3350-5c37f4c3ae2b5d651ef250994bdf3c482d39f24a41e177ffd3a4bc9bd2a13bc63</citedby><cites>FETCH-LOGICAL-c3350-5c37f4c3ae2b5d651ef250994bdf3c482d39f24a41e177ffd3a4bc9bd2a13bc63</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><linktohtml>$$Uhttps://www.sciencedirect.com/science/article/pii/0014579396010320$$EHTML$$P50$$Gelsevier$$Hfree_for_read</linktohtml><link.rule.ids>314,780,784,3549,27924,27925,45780</link.rule.ids><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8906863$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Tökés, Szilvia</creatorcontrib><creatorcontrib>Aradi, Janos</creatorcontrib><title>(s 4dU) 35: a novel, highly potent oligonucleotide inhibitor of the human immunodeficiency virus type 1 reverse transcriptase</title><title>FEBS letters</title><addtitle>FEBS Lett</addtitle><description>Oligodeoxycytidylates were converted to s
4dUMP-containing oligomers by treatment with liquid H
2S. The inhibitory potency of the modified oligonucleotides on human immunodeficiency virus type 1 reverse transcriptase depended on the chain length and on the percentage of modification. The most potent reverse transcriptase inhibitor was (s
4dU)
35. The inhibitory pattern was competitive, when either poly(A)·(dT)
16 or poly(C)·(dG)
16 was used as template-primer (variable substrate), suggesting that the free enzyme interacts with (s
4dU)
35. The
K
i
values were 3.0 and 2.2 nM in the presence of poly(A)·(dT)
16 and poly(C)·(dG)
16, respectively.</description><subject>AIDS, acquired immunodeficiency syndrome</subject><subject>AIDS/HIV</subject><subject>Antitemplate</subject><subject>Chromatography, High Pressure Liquid</subject><subject>Deoxyuracil Nucleotides - chemistry</subject><subject>DNA Primers</subject><subject>HIV Reverse Transcriptase - antagonists & inhibitors</subject><subject>HIV, human immunodeficiency virus</subject><subject>Human immunodeficiency virus</subject><subject>Inhibition</subject><subject>Na-PPi, Na-pyrophosphate</subject><subject>Oligo 4-thio-2′-deoxyuridylate</subject><subject>Oligonucleotides - chemistry</subject><subject>Oligonucleotides - pharmacology</subject><subject>Reverse transcriptase</subject><subject>Reverse Transcriptase Inhibitors - chemistry</subject><subject>Reverse Transcriptase Inhibitors - pharmacology</subject><subject>RT, reverse transcriptase</subject><subject>Structure-Activity Relationship</subject><subject>Sulfur - chemistry</subject><subject>TCA, trichloroacetic acid</subject><issn>0014-5793</issn><issn>1873-3468</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqNkE1rFTEUhoMo9Vr9BwpZSQuOJpPMR7oQtPSqUHBj1yGTnHQiM8mYZK7Mwv_uTO-lS3EVznk_TngQek3Je0po_YEQyouqEexC1JeEElYW5Ana0bZhBeN1-xTtHi3P0YuUfpJ1bqk4Q2etIHVbsx36c5EwN3eXmFVXWGEfDjC8w72774cFTyGDzzgM7j74WQ8QsjOAne9d53KIOFice8D9PCqP3TjOPhiwTjvwesEHF-eE8zIBpjjCAWICnKPySUc3ZZXgJXpm1ZDg1ek9R3f7mx_XX4vb71--XX-6LTRjFSkqzRrLNVNQdpWpKwq2rIgQvDOWad6WhglbcsUp0Kax1jDFOy06UyrKOl2zc_T22DvF8GuGlOXokoZhUB7CnGTTVmuQbUZ-NOoYUopg5RTdqOIiKZEbdbkhlRtSKdZhoy7JGntz6p-7Ecxj6IR51fdH_bcbYPmvTrm_-VxuwrYX9cN2O_TxWAQrrYODKNMDbDAugs7SBPfvn_4FrSOlqw</recordid><startdate>19961028</startdate><enddate>19961028</enddate><creator>Tökés, Szilvia</creator><creator>Aradi, Janos</creator><general>Elsevier B.V</general><scope>6I.</scope><scope>AAFTH</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7X8</scope></search><sort><creationdate>19961028</creationdate><title>(s 4dU) 35: a novel, highly potent oligonucleotide inhibitor of the human immunodeficiency virus type 1 reverse transcriptase</title><author>Tökés, Szilvia ; Aradi, Janos</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c3350-5c37f4c3ae2b5d651ef250994bdf3c482d39f24a41e177ffd3a4bc9bd2a13bc63</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>AIDS, acquired immunodeficiency syndrome</topic><topic>AIDS/HIV</topic><topic>Antitemplate</topic><topic>Chromatography, High Pressure Liquid</topic><topic>Deoxyuracil Nucleotides - chemistry</topic><topic>DNA Primers</topic><topic>HIV Reverse Transcriptase - antagonists & inhibitors</topic><topic>HIV, human immunodeficiency virus</topic><topic>Human immunodeficiency virus</topic><topic>Inhibition</topic><topic>Na-PPi, Na-pyrophosphate</topic><topic>Oligo 4-thio-2′-deoxyuridylate</topic><topic>Oligonucleotides - chemistry</topic><topic>Oligonucleotides - pharmacology</topic><topic>Reverse transcriptase</topic><topic>Reverse Transcriptase Inhibitors - chemistry</topic><topic>Reverse Transcriptase Inhibitors - pharmacology</topic><topic>RT, reverse transcriptase</topic><topic>Structure-Activity Relationship</topic><topic>Sulfur - chemistry</topic><topic>TCA, trichloroacetic acid</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Tökés, Szilvia</creatorcontrib><creatorcontrib>Aradi, Janos</creatorcontrib><collection>ScienceDirect Open Access Titles</collection><collection>Elsevier:ScienceDirect:Open Access</collection><collection>Medline</collection><collection>MEDLINE</collection><collection>MEDLINE (Ovid)</collection><collection>MEDLINE</collection><collection>MEDLINE</collection><collection>PubMed</collection><collection>CrossRef</collection><collection>MEDLINE - Academic</collection><jtitle>FEBS letters</jtitle></facets><delivery><delcategory>Remote Search Resource</delcategory><fulltext>fulltext</fulltext></delivery><addata><au>Tökés, Szilvia</au><au>Aradi, Janos</au><format>journal</format><genre>article</genre><ristype>JOUR</ristype><atitle>(s 4dU) 35: a novel, highly potent oligonucleotide inhibitor of the human immunodeficiency virus type 1 reverse transcriptase</atitle><jtitle>FEBS letters</jtitle><addtitle>FEBS Lett</addtitle><date>1996-10-28</date><risdate>1996</risdate><volume>396</volume><issue>1</issue><spage>43</spage><epage>46</epage><pages>43-46</pages><issn>0014-5793</issn><eissn>1873-3468</eissn><abstract>Oligodeoxycytidylates were converted to s
4dUMP-containing oligomers by treatment with liquid H
2S. The inhibitory potency of the modified oligonucleotides on human immunodeficiency virus type 1 reverse transcriptase depended on the chain length and on the percentage of modification. The most potent reverse transcriptase inhibitor was (s
4dU)
35. The inhibitory pattern was competitive, when either poly(A)·(dT)
16 or poly(C)·(dG)
16 was used as template-primer (variable substrate), suggesting that the free enzyme interacts with (s
4dU)
35. The
K
i
values were 3.0 and 2.2 nM in the presence of poly(A)·(dT)
16 and poly(C)·(dG)
16, respectively.</abstract><cop>England</cop><pub>Elsevier B.V</pub><pmid>8906863</pmid><doi>10.1016/0014-5793(96)01032-0</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0014-5793 |
| ispartof | FEBS letters, 1996-10, Vol.396 (1), p.43-46 |
| issn | 0014-5793 1873-3468 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78517736 |
| source | ScienceDirect Journals |
| subjects | AIDS, acquired immunodeficiency syndrome AIDS/HIV Antitemplate Chromatography, High Pressure Liquid Deoxyuracil Nucleotides - chemistry DNA Primers HIV Reverse Transcriptase - antagonists & inhibitors HIV, human immunodeficiency virus Human immunodeficiency virus Inhibition Na-PPi, Na-pyrophosphate Oligo 4-thio-2′-deoxyuridylate Oligonucleotides - chemistry Oligonucleotides - pharmacology Reverse transcriptase Reverse Transcriptase Inhibitors - chemistry Reverse Transcriptase Inhibitors - pharmacology RT, reverse transcriptase Structure-Activity Relationship Sulfur - chemistry TCA, trichloroacetic acid |
| title | (s 4dU) 35: a novel, highly potent oligonucleotide inhibitor of the human immunodeficiency virus type 1 reverse transcriptase |
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