A simple in vitro model to test the efficacy of antimicrobial agents released from dressings

A new model for in vitro evaluation of an activity of antibacterial agents released from delivery systems containing antibiotics or antiseptics was developed. The model was composed of two layers of agar gel of two different concentrations in PBS. In the upper gel, the wells were cut and filled with...

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Bibliographic Details
Published in:Journal of pharmacological and toxicological methods 1996-10, Vol.36 (2), p.73-76
Main Authors: Grzybowski, Jacek, Antos, Malgorzata, Trafny, Elżbieta A.
Format: Article
Language:English
Subjects:
Agar - chemistry
Agar - metabolism
Amikacin - metabolism
Amikacin - pharmacology
Animals
Anti-Bacterial Agents - pharmacology
Anti-Infective Agents, Local - pharmacology
Antimicrobial agent
Bandages - standards
Collagen - chemistry
Collagen - metabolism
Collagen dressing
Culture Media
Doxycycline - metabolism
Doxycycline - pharmacology
Drug Delivery Systems
Gels
in vitro test
Microbial Sensitivity Tests
Polyurethanes - metabolism
Porifera
Pseudomonas aeruginosa
Pseudomonas aeruginosa - drug effects
Pseudomonas aeruginosa - metabolism
Silver Sulfadiazine - metabolism
Silver Sulfadiazine - pharmacology
Staphylococcus aureus
Staphylococcus aureus - drug effects
Staphylococcus aureus - metabolism
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Summary:A new model for in vitro evaluation of an activity of antibacterial agents released from delivery systems containing antibiotics or antiseptics was developed. The model was composed of two layers of agar gel of two different concentrations in PBS. In the upper gel, the wells were cut and filled with polyurethane sponges saturated with bacterial broth culture. The sponges were then covered with tested dressings containing antibacterial agent. The model was tested by using two bacterial strains, Pseudomonas aeruginosa or Staphylococcus aureus and experimental collagen dressings with doxycyclin, amikacin, or with silver sulfadiazine. The number of colony-forming units (CFU) in the polyurethane sponges was significantly lower under the dressings with antibacterial agents as compared to the control (the same dressing without antimicrobials) during 3 days of observation. The new in vitro model can be recommended for examination of different antibacterial delivery systems instead of experimentally infected wounds in laboratory animals or instead of the other in vitro models.
ISSN:1056-8719
1873-488X
DOI:10.1016/S1056-8719(96)00076-7