Stimulation by Retinoids of the Natriuretic Peptide System of Osteoblastic MC3T3-E1 Cells

• Published in: Biochemical and biophysical research communications 1996-11, Vol.228 (1), p.182-186
• Main Authors: Inoue, Atsuto, Otsuka, Eri, Hiruma, Yoshiharu, Hirose, Shigehisa, Furuya, Mayumi, Tanaka, Shoji, Hagiwara, Hiromi
• Format: Article
• Language: English
• Subjects: Animals; Atrial Natriuretic Factor - metabolism; Binding Sites; Cell Line; Guanylate Cyclase - metabolism; Mice; Natriuretic Peptide, C-Type; Osteoblasts - drug effects; Osteoblasts - metabolism; Proteins - metabolism; Receptors, Atrial Natriuretic Factor - metabolism; Receptors, Retinoic Acid - genetics; Receptors, Retinoic Acid - metabolism; Retinoid X Receptors; RNA, Messenger - genetics; RNA, Messenger - metabolism; Transcription Factors - genetics; Transcription Factors - metabolism; Tretinoin - pharmacology
• ISSN: 0006-291X; 1090-2104
• DOI: 10.1006/bbrc.1996.1636

The effects were examined of treatment with retinoids of osteoblastic MC3T3-E1 cells on the natriuretic peptide system that promotes the differentiation of osteoblastic cells. Northern blot analysis revealed high levels of mRNA for the retinoid X receptor β (RXRβ) and moderate levels of mRNAs for retinoic acid receptors α (RARα) and γ (RARγ). Exposure of MC3T3-E1 cells to 1 μM retinoid caused increases in the levels of C-type natriuretic peptide (CNP) and natriuretic peptide receptor-C (NPR-C). The activity of natriuretic peptide receptor-B (NPR-B) was unchanged after the addition of retinoid to the culture system. These results suggest that retinoids might influence the metabolism of osteoblastic cells through regulation of the natriuretic peptide system.