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Suppression of fibroblast cell growth by overexpression of LIM-kinase 1

LIM-kinase 1 (LIMK1) is a serine/threonine kinase containing two LIM motifs at the N-terminus. The functional role of LIMKI has remained unknown. In this study, we examined the role of LIMK1 in cell growth of fibroblasts. Induced expression of LIMK1 in NIH3T3 cells led to growth retardation. Transfe...

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Bibliographic Details
Published in:FEBS letters 1996-10, Vol.396 (1), p.81-86
Main Authors: Higuchi, Osamu, Baeg, Gyeong-Hun, Akiyama, Tetsu, Mizuno, Kensaku
Format: Article
Language:English
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Summary:LIM-kinase 1 (LIMK1) is a serine/threonine kinase containing two LIM motifs at the N-terminus. The functional role of LIMKI has remained unknown. In this study, we examined the role of LIMK1 in cell growth of fibroblasts. Induced expression of LIMK1 in NIH3T3 cells led to growth retardation. Transfection of LIMK1 sense cDNA into NIH3T3 and H-ras-transformed FYJ10 fibroblasts significantly suppressed colony formation of these cells. In contrast, transfection with LIMK1 antisense cDNA strongly stimulated colony formation of the NIH3T3 cells. These findings suggest that LIMK1 functions as a negative regulator of fibroblast cell growth, and may play a role in tumor suppression.
ISSN:0014-5793
1873-3468
DOI:10.1016/0014-5793(96)01072-1