Growth and Transparency in the Lens, an Epithelial Tissue, Stimulated by Pulses of PDGF

The rat lens undergoes dramatic growth during early postnatal development. Lens weight increased by a factor of 23 in 26 days. Growth rate per day oscillated between 0 and 87 percent. A new culture system was designed to study the oscillations in growth during development. Lens growth and transparen...

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Bibliographic Details
Published in:Science (American Association for the Advancement of Science) 1988-11, Vol.242 (4879), p.777-779
Main Authors: Brewitt, Barbara, Clark, John I.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Cell growth
Cells
Crystalline lens
Developmental biology
Drug Administration Schedule
Epithelial cells
Epithelium
Epithelium - physiology
Eye and associated structures. Visual pathways and centers. Vision
Eye lens
Eyes & eyesight
Fibroblast growth factors
Fundamental and applied biological sciences. Psychology
Hormones
In Vitro Techniques
Laboratory rats
Lens, Crystalline - anatomy & histology
Lens, Crystalline - growth & development
Neuroglia
Optical lenses
Organ Size
Periodicity
Photographic lenses
Physical growth
Platelet-derived growth factor
Platelet-Derived Growth Factor - administration & dosage
Platelet-Derived Growth Factor - pharmacology
Rats
Rats as laboratory animals
Rodents
Time Factors
Vertebrates: nervous system and sense organs
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Summary:The rat lens undergoes dramatic growth during early postnatal development. Lens weight increased by a factor of 23 in 26 days. Growth rate per day oscillated between 0 and 87 percent. A new culture system was designed to study the oscillations in growth during development. Lens growth and transparency in vitro required pulsatile delivery of platelet-derived growth factor (PDGF) in HL-1 serum-free medium. Continuous delivery of HL-1 medium with PDGF or pulsatile delivery of HL-1 medium without PDGF resulted in lens opacity and no growth. These results provide direct evidence that PDGF stimulates an epithelial tissue and that oscillations in growth occur during normal development of the rat lens.
ISSN:0036-8075
1095-9203
DOI:10.1126/science.3187521