THE EFFECT OF DIFFERENT MICROTITRE PLATES ON THE ENZYME-LINKED IMMUNOSORBENT ASSAY (ELISA) FOR ANTICARDIOLIPIN ANTIBODIES (ACAs)

• Published in: Thrombosis research 1996-11, Vol.84 (3), p.217-222
• Main Authors: Hughes, J.Richard, Davies, J.Andrew, Prentice, Colin R.M.
• Format: Article
• Language: English
• Subjects: antibodies; Antibodies, Anticardiolipin - analysis; anticardiolipin; Biological and medical sciences; Enzyme-Linked Immunosorbent Assay - methods; Humans; lupus anticoagulant; Medical sciences; Reproducibility of Results; Sarcoidosis. Granulomatous diseases of unproved etiology. Connective tissue diseases. Elastic tissue diseases. Vasculitis; systemic lupus erythematosus
• ISSN: 0049-3848; 1879-2472
• DOI: 10.1016/0049-3848(96)00180-6

Studies have shown an association between the presence of ACAs, measured by an ELISA, and thrombosis, foetal loss and thrombocytopenia [1–7]. Most patients with the syndrome have systemic lupus erythematosus (SLE) or a related autoimmune disease [8]. In studies of the rates of thrombotic episodes in patients with the lupus anticoagulant (LAC) and, or ACAs associated with SLE and non-SLE diseases there is considerable variation. For instance, investigations of women with a poor obstetric history: 13.1% had ACAs [4], 42.4% [3], 81.5% [5], others have found a lower frequency of habitual aborters with the LAC and, or ACAs arguing that ACAs play only a minor role in spontaneous abortion [9]. This paper reports the results of an investigation into how the choice of brand name microtitre plates affects the result of the ACA ELISA.