Characterization of transforming growth factor-β-resistant subclones isolated from a transforming growth factor-β-sensitive human colon carcinoma cell line

Previous work indicated that transforming growth factor-beta (TGF-beta) elicits proliferation-inhibitory effects in the human colon carcinoma cell line MOSER. This paper describes the isolation and characterization of spontaneously arising subclones from this TGF-beta-sensitive parental line which w...

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Bibliographic Details
Published in:Cancer research (Chicago, Ill.) Ill.), 1988-12, Vol.48 (24), p.7120-7125
Main Authors: MULDER, K. M, RAMEY, M. K, HOOSEIN, N. M, LEVINE, A. E, HINSHAW, X. H, BRATTAIN, D. E, BRATTAIN, M. G
Format: Article
Language:English
Subjects:
Biological and medical sciences
Cell Line
Clone Cells - drug effects
Colonic Neoplasms - pathology
Dimethylformamide - pharmacology
Drug Resistance
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Medical sciences
Oncogenes
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Transforming Growth Factors - pharmacology
Tretinoin - pharmacology
Tumors
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Summary:Previous work indicated that transforming growth factor-beta (TGF-beta) elicits proliferation-inhibitory effects in the human colon carcinoma cell line MOSER. This paper describes the isolation and characterization of spontaneously arising subclones from this TGF-beta-sensitive parental line which were relatively refractory to the inhibitory effects of TGF-beta. While the parental cell line responded to TGF-beta with an inhibition of cellular proliferation in monolayer culture and in soft agarose, an increase in extracellular fibronectin, and a down-regulation of c-myc protooncogene expression, these responses were absent or attenuated in the sublines. However, the resistant clones retained the ability to specifically bind TGF-beta. N,N-Dimethylformamide and retinoic acid, two other agents associated with induction of a partial differentiation-like response in the MOSER parental cells (similar to that elicited by TGF-beta), inhibited the monolayer proliferation of both the parental cells and the TGF-beta-resistant sublines. Thus, the refractoriness observed in the isolated clones was relatively specific for TGF-beta.
ISSN:0008-5472
1538-7445