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An improved method for the detection of Tospoviruses using the polymerase chain reaction
A reverse transcription-polymerase chain reaction (RT-PCR)-based assay for the detection of tomato spotted wilt virus (TSWV) has been improved and extended to enable the detection of additional Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one p...
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| Published in: | Journal of virological methods 1996-03, Vol.57 (1), p.109-115 |
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| Main Authors: | , , |
| Format: | Article |
| Language: | English |
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| cited_by | cdi_FETCH-LOGICAL-c417t-57b6665162ad1f9ab20b25957c3e4cf604e4f477d27e5fe6b2b926c1a5fa29353 |
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| cites | cdi_FETCH-LOGICAL-c417t-57b6665162ad1f9ab20b25957c3e4cf604e4f477d27e5fe6b2b926c1a5fa29353 |
| container_end_page | 115 |
| container_issue | 1 |
| container_start_page | 109 |
| container_title | Journal of virological methods |
| container_volume | 57 |
| creator | Mumford, R.A. Barker, I. Wood, K.R. |
| description | A reverse transcription-polymerase chain reaction (RT-PCR)-based assay for the detection of tomato spotted wilt virus (TSWV) has been improved and extended to enable the detection of additional
Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (INSV) and another which detects all
Tospoviruses tested, including TSWV, INSV, tomato chlorotic spot virus and groundnut ringspot virus. An improved, rapid RNA extraction method is also described. |
| doi_str_mv | 10.1016/0166-0934(95)01975-8 |
| format | article |
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Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (INSV) and another which detects all
Tospoviruses tested, including TSWV, INSV, tomato chlorotic spot virus and groundnut ringspot virus. An improved, rapid RNA extraction method is also described.</description><identifier>ISSN: 0166-0934</identifier><identifier>EISSN: 1879-0984</identifier><identifier>DOI: 10.1016/0166-0934(95)01975-8</identifier><identifier>PMID: 8919828</identifier><identifier>CODEN: JVMEDH</identifier><language>eng</language><publisher>London: Elsevier B.V</publisher><subject>Base Sequence ; Biological and medical sciences ; DNA Primers ; DNA, Viral - analysis ; Fundamental and applied biological sciences. Psychology ; Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control ; Groundnut ringspot virus ; Impatiens necrotic spot virus ; Microbiology ; Molecular Sequence Data ; Phytopathology. Animal pests. Plant and forest protection ; Plant viral diagnostics ; Plant viruses and viroids ; Polymerase Chain Reaction - methods ; RNA, Viral - analysis ; RT-PCR ; Techniques used in virology ; Tomato chlorotic spot virus ; Tomato spotted wilt virus ; Tospovirus - genetics ; Tospovirus - isolation & purification ; Tospoviruses ; Virology</subject><ispartof>Journal of virological methods, 1996-03, Vol.57 (1), p.109-115</ispartof><rights>1996</rights><rights>1996 INIST-CNRS</rights><lds50>peer_reviewed</lds50><woscitedreferencessubscribed>false</woscitedreferencessubscribed><citedby>FETCH-LOGICAL-c417t-57b6665162ad1f9ab20b25957c3e4cf604e4f477d27e5fe6b2b926c1a5fa29353</citedby><cites>FETCH-LOGICAL-c417t-57b6665162ad1f9ab20b25957c3e4cf604e4f477d27e5fe6b2b926c1a5fa29353</cites></display><links><openurl>$$Topenurl_article</openurl><openurlfulltext>$$Topenurlfull_article</openurlfulltext><thumbnail>$$Tsyndetics_thumb_exl</thumbnail><link.rule.ids>314,780,784,27924,27925</link.rule.ids><backlink>$$Uhttp://pascal-francis.inist.fr/vibad/index.php?action=getRecordDetail&idt=3042852$$DView record in Pascal Francis$$Hfree_for_read</backlink><backlink>$$Uhttps://www.ncbi.nlm.nih.gov/pubmed/8919828$$D View this record in MEDLINE/PubMed$$Hfree_for_read</backlink></links><search><creatorcontrib>Mumford, R.A.</creatorcontrib><creatorcontrib>Barker, I.</creatorcontrib><creatorcontrib>Wood, K.R.</creatorcontrib><title>An improved method for the detection of Tospoviruses using the polymerase chain reaction</title><title>Journal of virological methods</title><addtitle>J Virol Methods</addtitle><description>A reverse transcription-polymerase chain reaction (RT-PCR)-based assay for the detection of tomato spotted wilt virus (TSWV) has been improved and extended to enable the detection of additional
Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (INSV) and another which detects all
Tospoviruses tested, including TSWV, INSV, tomato chlorotic spot virus and groundnut ringspot virus. An improved, rapid RNA extraction method is also described.</description><subject>Base Sequence</subject><subject>Biological and medical sciences</subject><subject>DNA Primers</subject><subject>DNA, Viral - analysis</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</subject><subject>Groundnut ringspot virus</subject><subject>Impatiens necrotic spot virus</subject><subject>Microbiology</subject><subject>Molecular Sequence Data</subject><subject>Phytopathology. Animal pests. Plant and forest protection</subject><subject>Plant viral diagnostics</subject><subject>Plant viruses and viroids</subject><subject>Polymerase Chain Reaction - methods</subject><subject>RNA, Viral - analysis</subject><subject>RT-PCR</subject><subject>Techniques used in virology</subject><subject>Tomato chlorotic spot virus</subject><subject>Tomato spotted wilt virus</subject><subject>Tospovirus - genetics</subject><subject>Tospovirus - isolation & purification</subject><subject>Tospoviruses</subject><subject>Virology</subject><issn>0166-0934</issn><issn>1879-0984</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1996</creationdate><recordtype>article</recordtype><recordid>eNqFkU1rFTEUhoMo7bX6DyxkIaKL0SSTz41Qiv2AgpsK7kImc-KNzExuk5kL_ffmfnCXdREO5DzvIXkOQh8o-UoJld_qkQ0xLf9sxBdCjRKNfoVWVCtTrzV_jVYn5By9LeUvIUSotj1DZ9pQo5leod9XE47jJqct9HiEeZ16HFLG8xpwDzP4OaYJp4AfU9mkbcxLgYKXEqc_e2aThucRsiuA_drFCWdw-8w79Ca4ocD7Y71Av25-PF7fNQ8_b--vrx4az6maG6E6KaWgkrmeBuM6RjomjFC-Be6DJBx44Er1TIEIIDvWGSY9dSI4ZlrRXqBPh7n1D08LlNmOsXgYBjdBWopVWrRcUPVfkApNDJekgvwA-pxKyRDsJsfR5WdLid2ZtzutdqfVGmH35q2uscvj_KUboT-Fjqpr_-Ox74p3Q8hu8rGcsJZwpgWr2PcDBlXaNkK2xUeYPPQx123YPsWX3_EPPJ2fYQ</recordid><startdate>19960301</startdate><enddate>19960301</enddate><creator>Mumford, R.A.</creator><creator>Barker, I.</creator><creator>Wood, K.R.</creator><general>Elsevier B.V</general><general>Elsevier</general><scope>IQODW</scope><scope>CGR</scope><scope>CUY</scope><scope>CVF</scope><scope>ECM</scope><scope>EIF</scope><scope>NPM</scope><scope>AAYXX</scope><scope>CITATION</scope><scope>7T7</scope><scope>7U9</scope><scope>8FD</scope><scope>C1K</scope><scope>FR3</scope><scope>H94</scope><scope>P64</scope><scope>7X8</scope></search><sort><creationdate>19960301</creationdate><title>An improved method for the detection of Tospoviruses using the polymerase chain reaction</title><author>Mumford, R.A. ; Barker, I. ; Wood, K.R.</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c417t-57b6665162ad1f9ab20b25957c3e4cf604e4f477d27e5fe6b2b926c1a5fa29353</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1996</creationdate><topic>Base Sequence</topic><topic>Biological and medical sciences</topic><topic>DNA Primers</topic><topic>DNA, Viral - analysis</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control</topic><topic>Groundnut ringspot virus</topic><topic>Impatiens necrotic spot virus</topic><topic>Microbiology</topic><topic>Molecular Sequence Data</topic><topic>Phytopathology. Animal pests. 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Tospoviruses. In addition to TSWV-specific primers, two further pairs of primers have been designed, one pair which specifically detects impatiens necrotic spot virus (INSV) and another which detects all
Tospoviruses tested, including TSWV, INSV, tomato chlorotic spot virus and groundnut ringspot virus. An improved, rapid RNA extraction method is also described.</abstract><cop>London</cop><cop>Amsterdam</cop><cop>New York, NY</cop><pub>Elsevier B.V</pub><pmid>8919828</pmid><doi>10.1016/0166-0934(95)01975-8</doi><tpages>7</tpages></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0166-0934 |
| ispartof | Journal of virological methods, 1996-03, Vol.57 (1), p.109-115 |
| issn | 0166-0934 1879-0984 |
| language | eng |
| recordid | cdi_proquest_miscellaneous_78534517 |
| source | ScienceDirect Freedom Collection |
| subjects | Base Sequence Biological and medical sciences DNA Primers DNA, Viral - analysis Fundamental and applied biological sciences. Psychology Generalities. Techniques. Transmission, epidemiology, ecology. Antiviral substances, control Groundnut ringspot virus Impatiens necrotic spot virus Microbiology Molecular Sequence Data Phytopathology. Animal pests. Plant and forest protection Plant viral diagnostics Plant viruses and viroids Polymerase Chain Reaction - methods RNA, Viral - analysis RT-PCR Techniques used in virology Tomato chlorotic spot virus Tomato spotted wilt virus Tospovirus - genetics Tospovirus - isolation & purification Tospoviruses Virology |
| title | An improved method for the detection of Tospoviruses using the polymerase chain reaction |
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