An expressed sequence tag analysis of a full-length, spliced-leader cDNA library from Leishmania major promastigotes

The generation of expressed sequence tags (ESTs) has proven to be an efficient method for gene identification and classification in protozoan parasites such as Plasmodium falciparum, Trypanosoma brucei and Toxoplasma gondii. The presence of a 5' spliced-leader sequence on most trypanosomatid mR...

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Bibliographic Details
Published in:Molecular and biochemical parasitology 1996-02, Vol.76 (1), p.345-348
Main Authors: Levick, Mark P., Blackwell, Jenefer M., Connor, Vivienne, Coulson, Richard M.R., Miles, Alistair, Smith, Heather E., Wan, Kiew-Lian, Ajioka, James W.
Format: Article
Language:English
Subjects:
Animals
cDNA
Cloning, Molecular
DNA, Complementary - genetics
DNA, Protozoan
Expressed sequence tag
Gene Expression
Gene Library
Leishmania major
Leishmania major - genetics
Molecular Sequence Data
Promastigote
Protein Methyltransferases - genetics
Protein Sorting Signals - analysis
Sequence Tagged Sites
Triose-Phosphate Isomerase - genetics
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Summary:The generation of expressed sequence tags (ESTs) has proven to be an efficient method for gene identification and classification in protozoan parasites such as Plasmodium falciparum, Trypanosoma brucei and Toxoplasma gondii. The presence of a 5' spliced-leader sequence on most trypanosomatid mRNAs and some nematode mRNAs has been used to identify the 5' terminus in some cDNAs from a conventional cDNA library and to construct a spliced-leader cDNA library. Here we report an EST analysis of a directionally cloned, full-length, spliced-leader cDNA constructed from promastigotes of Leishmania major.
ISSN:0166-6851
1872-9428
DOI:10.1016/0166-6851(95)02569-3