New cosmid vectors for library construction, chromosome walking and restriction mapping in filamentous fungi

New cosmid vectors were constructed for the ascomycete fungus, Magnaporthe grisea and the basidiomycete fungus, Ustilago maydis. These vectors are capable of transforming M. grisea at frequencies of up to 5 transformants/μg linear DNA and U. maydis at up to 25 transformants/μg circular DNA for integ...

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Bibliographic Details
Published in:Gene 1996-10, Vol.176 (1), p.93-96
Main Authors: An, Zhiqiang, Farman, Mark L., Budde, Allen, Taura, Satoru, Leong, Sally A.
Format: Article
Language:English
Subjects:
Ascomycetes
Ascomycota - genetics
Base Sequence
Basidiomycetes
Chromosome Walking
construction
Cosmids
DNA, Bacterial
genbank/l76723
Gene Library
genetic markers
genetic transformation
Genetic Vectors
Magnaporthe grisea
Molecular Sequence Data
nucleotide sequences
Promoter
restriction enzyme mediated integration
Restriction Mapping
Transformant
Transformation, Genetic
Ustilago - genetics
Ustilago maydis
Ustilago zeae
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Summary:New cosmid vectors were constructed for the ascomycete fungus, Magnaporthe grisea and the basidiomycete fungus, Ustilago maydis. These vectors are capable of transforming M. grisea at frequencies of up to 5 transformants/μg linear DNA and U. maydis at up to 25 transformants/μg circular DNA for integrative transformation. In addition, 2800 transformants/μg DNA are possible when using an autonomously replicating vector. Since the promoters used in these vectors function in other ascomycete and basidiomycete fungi, we anticipate that these vectors will be widely applicable.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(96)00225-9