Induction of astrocyte argininosuccinate synthetase and argininosuccinate lyase by dibutyryl cyclic AMP and dexamethasone

Arginine is an intermediate in the elimination of excess nitrogen and is the substrate for nitric oxide synthesis. Arginine synthesis has been reported in brain tissue. We have studied the activity of the arginine biosynthetic enzymes argininosuccinate synthetase and argininosuccinate lyase in dexam...

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Bibliographic Details
Published in:Neurochemical research 1996-10, Vol.21 (10), p.1161-1165
Main Authors: JACKSON, M. J, ZIELKE, H. R, ZIELKE, C. L
Format: Article
Language:English
Subjects:
Adenylyl Cyclases - biosynthesis
Animals
Animals, Newborn
Argininosuccinate Lyase - biosynthesis
Argininosuccinate Synthase - biosynthesis
Astrocytes - drug effects
Astrocytes - enzymology
Biological and medical sciences
Bucladesine - pharmacology
Cells, Cultured
Dexamethasone - pharmacology
Enzyme Induction
Fundamental and applied biological sciences. Psychology
Glucagon - pharmacology
Glucocorticoids - pharmacology
Isolated neuron and nerve. Neuroglia
Rats
Reproducibility of Results
Vertebrates: nervous system and sense organs
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Summary:Arginine is an intermediate in the elimination of excess nitrogen and is the substrate for nitric oxide synthesis. Arginine synthesis has been reported in brain tissue. We have studied the activity of the arginine biosynthetic enzymes argininosuccinate synthetase and argininosuccinate lyase in dexamethasone and/or dibutyryl cyclic AMP treated rat astrocyte cultures. Argininosuccinate lyase activity was stimulated by treatment with either effector and an additive effect was obtained when both agents were added simultaneously. Argininosuccinate synthetase was also increased in dexamethasone treated astrocytes. The effect of dibutyryl cyclic AMP on argininosuccinate synthetase was variable, suggesting a role for additional factors in its regulation as compared to argininosuccinate lyase. Regulation of arginine synthesis in astrocytes may be important to insure that arginine is not limiting for nitric oxide synthesis in neural tissue.
ISSN:0364-3190
1573-6903
DOI:10.1007/BF02532390