Effects of ischaemia and preservation on the ultrastructure of the bronchiolar epithelium : A quantitative electron microscopic study of human and canine lungs

In ten cases of clinical human single-lung transplantation, the nontransplanted Euro-Collins-preserved contralateral lungs were examined using electron microscopy to determine the effects of ischaemia on the bronchiolar epithelium. Existing structural damage at the time of transplantation was charac...

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Bibliographic Details
Published in:Virchows Archiv : an international journal of pathology 1996-10, Vol.429 (2-3), p.109-118
Main Authors: STOLTE, N, FEHRENBACH, H, OCHS, M, SCHMIEDL, A, HIRT, S. W, WAHLERS, T, RICHTER, J
Format: Article
Language:English
Subjects:
Adolescent
Adult
Animals
Biological and medical sciences
Bronchi - blood supply
Bronchi - ultrastructure
Dogs
Epithelium - blood supply
Epithelium - ultrastructure
Female
Humans
Hypertonic Solutions
Ischemia - pathology
Male
Medical sciences
Microscopy, Electron
Microscopy, Electron, Scanning
Middle Aged
Mitochondria - ultrastructure
Organ Preservation
Pneumology
Respiratory system : syndromes and miscellaneous diseases
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Summary:In ten cases of clinical human single-lung transplantation, the nontransplanted Euro-Collins-preserved contralateral lungs were examined using electron microscopy to determine the effects of ischaemia on the bronchiolar epithelium. Existing structural damage at the time of transplantation was characterized using this approach, and nine nonpreserved canine single lungs were also investigated to identify the impact of ischaemia. The study revealed a significant correlation between the duration of ischaemia and the mitochondrial surface-to-volume ratio, which can serve as a morphometric criterion for mitochondrial damage, in canine lungs. However, this correlation was not found in the human donor lungs. Further examination of human donor lungs showed slight to moderate damage to the endoplasmic reticulum and nuclear chromatin. In addition, various degrees of damage to mitochondrial structure, ranging from inconspicuous to severe, were found. The mitochondrial surface-to-volume ratio can be considered to be a suitable criterion for the quantification of ischaemic damage of the bronchiolar epithelium under experimental conditions. Ultrastructural analysis of human donor lungs revealed intact bronchiolar epithelial cell structures at the time of transplantation, reflecting adequate organ preservation with Euro-Collins solution.
ISSN:0945-6317
1432-2307
DOI:10.1007/BF00192433