Mitochondrial DNA polymerase from Drosophila melanogaster embryos: kinetics, processivity, and fidelity of DNA polymerization

The mitochondrial DNA polymerase from embryos of Drosophila melanogaster has been examined with regard to template-primer utilization, processivity, and fidelity of nucleotide polymerization. The enzyme replicates predominantly single-stranded and double-stranded DNAs: the rate of DNA synthesis is g...

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Bibliographic Details
Published in:Biochemistry (Easton) 1988-08, Vol.27 (16), p.6046-6054
Main Authors: Wernette, Catherine M, Conway, Michael C, Kaguni, Laurie S
Format: Article
Language:English
Subjects:
ADN
Analytical, structural and metabolic biochemistry
ANIMAL EMBRYOS
Animals
Biological and medical sciences
DNA
DNA Polymerase III - metabolism
DNA, Mitochondrial - biosynthesis
DNA-Directed DNA Polymerase - metabolism
DROSOPHILA
Drosophila melanogaster
Drosophila melanogaster - enzymology
Drosophilidae
EMBRIONES ANIMALES
Embryo, Nonmammalian - enzymology
EMBRYON ANIMAL
Enzymes and enzyme inhibitors
Fundamental and applied biological sciences. Psychology
Kinetics
MITOCHONDRIA
Mitochondria - enzymology
MITOCHONDRIE
MITOCONDRIA
Substrate Specificity
Transferases
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Summary:The mitochondrial DNA polymerase from embryos of Drosophila melanogaster has been examined with regard to template-primer utilization, processivity, and fidelity of nucleotide polymerization. The enzyme replicates predominantly single-stranded and double-stranded DNAs: the rate of DNA synthesis is greatest on the gapped homopolymeric template poly(dA).oligo(dT), while the highest substrate specificity is observed on single-stranded DNA templates of natural DNA sequence. Kinetic experiments and direct physical analysis of DNA synthetic products indicate that the Drosophila DNA polymerase gamma polymerizes nucleotides by a quasi-processive mechanism. The mitochondrial enzyme demonstrates a high degree of accuracy in nucleotide incorporation which is nearly identical with that of the replicative DNA polymerase alpha from Drosophila embryos. Thus, the catalytic properties of the near-homogeneous Drosophila DNA polymerase gamma are consistent with the in vivo requirements for mitochondrial DNA synthesis as described in a variety of animal systems.
ISSN:0006-2960
1520-4995
DOI:10.1021/bi00416a033