A Radioimmunoassay for African Catfish Growth Hormone: Validation and Effects of Substances Modulating the Release of Growth Hormone

A highly sensitive radioimmunoassay has been developed for measuring plasma growth hormone (GH) concentrations in the African catfish (Clarias gariepinus). The lower detection limit of the assay was 0.1 ng/ml and the standard curve had an ED50value of 0.5 ng/ml. The validity of the assay was establi...

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Bibliographic Details
Published in:General and comparative endocrinology 1996-11, Vol.104 (2), p.147-155
Main Authors: Lescroart, O., Roelants, I., Mikolajczyk, T., Bosma, P.T., Schulz, R.W., Kühn, E.R., Ollevier, F.
Format: Article
Language:English
Subjects:
Animals
Apomorphine - administration & dosage
Catfishes
Cells, Cultured
Clarias gariepinus
Cross Reactions
Dopamine Agonists - administration & dosage
Dose-Response Relationship, Drug
Female
Freshwater
Gonadotropin-Releasing Hormone - administration & dosage
Gonadotropin-Releasing Hormone - analogs & derivatives
Growth Hormone - analysis
Growth Hormone - drug effects
Growth Hormone - immunology
Growth Hormone - metabolism
Hormone Antagonists - pharmacology
Immune Sera - immunology
Immunohistochemistry
Injections, Intraperitoneal
Iodine Radioisotopes
Male
Pituitary Gland - anatomy & histology
Pituitary Gland - chemistry
Pituitary Gland - cytology
Pituitary Gland - immunology
Rabbits
Radioimmunoassay - methods
Reproducibility of Results
Sensitivity and Specificity
Sermorelin - pharmacology
Somatostatin - pharmacology
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Description
Summary:A highly sensitive radioimmunoassay has been developed for measuring plasma growth hormone (GH) concentrations in the African catfish (Clarias gariepinus). The lower detection limit of the assay was 0.1 ng/ml and the standard curve had an ED50value of 0.5 ng/ml. The validity of the assay was established and the effects of several neurotransmitters on the release of GH were examined.In vitroexperiments, using a static culture system for dispersed pituitary cells, demonstrated that the GH release in African catfish was affected by growth hormone-releasing hormone and somatostatin. Single intraperitoneal injections with a dopamine agonist, apomorphine, produced significant and dose-dependent increases in plasma GH levels. Unlike carp, goldfish, and tilapia, a super-active analogue of salmon gonadotrophin-releasing hormone did not alter plasma GH levels in African catfish.
ISSN:0016-6480
1095-6840
DOI:10.1006/gcen.1996.0157