A stress-sensitive chemokinergic neuronal pathway in the hypothalamo-pituitary system

• Published in: Neuroscience 1996-11, Vol.75 (1), p.133-142
• Main Authors: Sakamoto, Y, Koike, K, Kiyama, H, Konishi, K, Watanabe, K, Tsurufuji, S, Bicknell, R.J, Hirota, K, Miyake, A
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Chemokines, CXC; Chemotactic Factors - biosynthesis; Chemotactic Factors - genetics; Chemotactic Factors - metabolism; cytokine induced neutrophil chemoattractant; Fundamental and applied biological sciences. Psychology; Gene Expression Regulation; Growth Substances - biosynthesis; Growth Substances - genetics; Growth Substances - metabolism; Hormones and neuropeptides. Regulation; Hypophysectomy; hypophysis; Hypothalamo-Hypophyseal System - physiology; Hypothalamus. Hypophysis. Epiphysis. Urophysis; Immobilization; immobilization stress; Intercellular Signaling Peptides and Proteins; Male; Nerve Tissue Proteins - biosynthesis; Nerve Tissue Proteins - genetics; Nerve Tissue Proteins - metabolism; Neurons - drug effects; Neurons - physiology; paraventricular hypothalamic nucleus; Paraventricular Hypothalamic Nucleus - metabolism; Rats; Rats, Sprague-Dawley; RNA, Messenger - biosynthesis; Space life sciences; Specific Pathogen-Free Organisms; Stress, Physiological - physiopathology; supraoptic nucleus; Supraoptic Nucleus - metabolism; Vertebrates: endocrinology
• ISSN: 0306-4522; 1873-7544
• DOI: 10.1016/0306-4522(96)00252-7

Recently we found that cytokine-induced neutrophil chemoattractant influenced anterior pituitary hormone release in vitro. These observations prompted us to investigate the possibility of the existence of cytokine-induced neutrophil chemoattractant in the hypothalamus. Immunohistochemistry showed that cytokine-induced neutrophil chemoattractant-like immunoreactivity existed in the paraventricular hypothalamic nucleus, the supraoptic nucleus, both the internal and the external layers of the median eminence and the posterior pituitary. Since the paraventricular hypothalamic nucleus plays a pivotal role in response to stressful stimuli, we examined the effect of a single episode of immobilization stress on cytokine-induced neutrophil chemoattractant messenger RNA expression in the paraventricular hypothalamic nucleus. Immobilization stress induced strong hybridization signals of cytokine-induced neutrophil chemoattractant messenger RNA in the parvocellular and magnocellular subdivision of the paraventricular hypothalamic nucleus within 15 min, and cytokine-induced neutrophil chemoattractant-like immunostaining intensity in the posterior pituitary started to increase around the periphery of the posterior lobe at 30 min after stress and extended to the whole lobe at 1 h after stress. The increase in the serum cytokine-induced neutrophil chemoattractant in response to stress showed a kinetically biphasic pattern. A first phase occurred within 15 min which may be due to an immediate release of stored cytokine-induced neutrophil chemoattractant in the neurohypophysis, since hypophysectomy completely blocked this phase. A second phase may reflect the release of newly synthesized cytokine-induced neutrophil chemoattractant in the paraventricular hypothalamic nucleus and/or peripheral cytokine-induced neutrophil chemoattractant, since hypophysectomy could not reduce this phase. These data suggest that cytokine-induced neutrophil chemoattractant in the paraventricular hypothalamic nucleus was immediately synthesized in response to stress, and then released into the peripheral blood via the hypothalamo-neurohypophysial system, revealing the presence of a stress-sensitive chemokinergic neuronal pathway in the hypothalamo-pituitary system.