Interactions of the LIM-domain-binding factor Ldb1 with LIM homeodomain proteins

The LIM homeodomain (LIM-HD) proteins, which contain two tandem LIM domains followed by a homeodomain, are critical transcriptional regulators of embryonic development. The LIM domain is a conserved cysteine-rich zinc-binding motif found in LIM-HD and LMO (rhombotin or Ttg) proteins, cytoskeletal co...

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Bibliographic Details
Published in:Nature (London) 1996-11, Vol.384 (6606), p.270-272
Main Authors: AGULNICK, A. D, TAIRA, M, BREEN, J. J, TANAKA, T, DAWID, I. B, WESTPHAL, H
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Binding Sites
Biological and medical sciences
Cell differentiation, maturation, development, hematopoiesis
Cell physiology
Cloning
DNA-Binding Proteins - genetics
DNA-Binding Proteins - metabolism
Embryonic growth stage
Embryos
Fundamental and applied biological sciences. Psychology
Homeodomain Proteins - metabolism
Hybridization
Kinases
LIM Domain Proteins
LIM-Homeodomain Proteins
Localization
Mice
Molecular and cellular biology
Molecular Sequence Data
Protein Binding
Proteins
Recombinant Proteins - metabolism
Transcription Factors
Xenopus
Xenopus Proteins
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Summary:The LIM homeodomain (LIM-HD) proteins, which contain two tandem LIM domains followed by a homeodomain, are critical transcriptional regulators of embryonic development. The LIM domain is a conserved cysteine-rich zinc-binding motif found in LIM-HD and LMO (rhombotin or Ttg) proteins, cytoskeletal components, LIM kinases and other proteins. LIM domains are protein-protein interaction motifs, can inhibit binding of LIM-HD proteins to DNA and can negatively regulate LIM-HD protein function. How LIM domains exert these regulatory effects is not known. We have now isolated a new LIM-domain-binding factor, Ldb1, on the basis of its ability to interact with the LIM-HD protein Lhx1 (Lim1). High-affinity binding by Ldb1 requires paired LIM domains and is restricted to the related subgroup of LIM domains found in LIM-HD and LMO proteins. The highly conserved Xenopus Ldb protein XLdb1, interacts with Xlim-1, the Xenopus orthologue of Lhx1. When injected into Xenopus embryos, XLdb1 (or Ldb1) can synergize with Xlim-1 in the formation of partial secondary axes and in activation of the genes encoding goosecoid (gsc), chordin, NCAM and XCG7, demonstrating a functional as well as a physical interaction between the two proteins.
ISSN:0028-0836
1476-4687
DOI:10.1038/384270a0