Immunohistochemical detection of P53 overexpression in paraffin wax-embedded squamous cell carcinomas of cattle, horses, cats and dogs

One hundred and six squamous cell carcinomas (SCCs) of cattle, horses, cats and dogs were analysed immunohistochemically for overexpression of p53 protein. The monoclonal antibody pAb 240, which recognizes only mutant p53, was used. Of 41 bovine ocular SCCs, 26 (63·4%) showed p53 nuclear reactivity....

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Bibliographic Details
Published in:Journal of comparative pathology 1996-02, Vol.114 (2), p.205-210
Main Authors: Teifke, J.P., Löhr, C.V.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Carcinoma, Squamous Cell - chemistry
Carcinoma, Squamous Cell - pathology
Carcinoma, Squamous Cell - veterinary
Cat Diseases - metabolism
Cat Diseases - pathology
Cats
Cattle
Cattle Diseases - metabolism
Cattle Diseases - pathology
Dog Diseases - metabolism
Dog Diseases - pathology
Dogs
Female
Horse Diseases - metabolism
Horse Diseases - pathology
Horses
Host-tumor relations. Immunology. Biological markers
Male
Medical sciences
Paraffin Embedding
Skin Neoplasms - chemistry
Skin Neoplasms - pathology
Skin Neoplasms - veterinary
Tumor Suppressor Protein p53 - analysis
Tumor Suppressor Protein p53 - biosynthesis
Tumors
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Summary:One hundred and six squamous cell carcinomas (SCCs) of cattle, horses, cats and dogs were analysed immunohistochemically for overexpression of p53 protein. The monoclonal antibody pAb 240, which recognizes only mutant p53, was used. Of 41 bovine ocular SCCs, 26 (63·4%) showed p53 nuclear reactivity. All of six (100%) equine ocular SCCs and seven of nine (77·7%) SCCs of the equine penis or vulva gave positive reactions. In nine of 11 (81·8%) feline SCCs of the ear and in seven of 14 (50%) feline SCCs of other locations, p53 immunoreactivity was detected. Only seven of 25 (29·5%) canine cutaneous SCCs gave a positive reaction. Thus p53 antigen could be detected immunohistochemically in formalin-fixed and paraffin waxembedded tissues of SCCs of domestic animals. The results support the view that, as in man, p53 overexpression plays an important role in the development of most SCCs of the animal species studied. This was in particular true for feline SCCs of the ear and for bovine and equine ocular SCCs, which are assumed to be related to ultraviolet radiation.
ISSN:0021-9975
1532-3129
DOI:10.1016/S0021-9975(96)80010-7