Three‐dimensional visualization of Salmonella attachment to poultry skin using confocal scanning laser microscopy

K.Y. KIM, J.F. FRANK AND S.E. CRAVEN. 1996. The objective of this study was to locate the position of attached or entrapped Salmonella cells in poultry skin. Confocal scanning laser microscopy (CSLM) was used to obtain optical sections of intact poultry skin without artefacts associated with dehydra...

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Bibliographic Details
Published in:Letters in applied microbiology 1996-04, Vol.22 (4), p.280-282
Main Authors: Kim, K.Y., Frank, J.F., Craven, S.E.
Format: Article
Language:English
Subjects:
Animals
Bacterial Adhesion
Biotechnology
Image Processing, Computer-Assisted - methods
Microscopy, Confocal
Poultry - microbiology
Salmonella typhimurium - isolation & purification
Skin - microbiology
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Summary:K.Y. KIM, J.F. FRANK AND S.E. CRAVEN. 1996. The objective of this study was to locate the position of attached or entrapped Salmonella cells in poultry skin. Confocal scanning laser microscopy (CSLM) was used to obtain optical sections of intact poultry skin without artefacts associated with dehydration and other sample preparation techniques. A technique was developed to prevent compression of the poultry skin during CSLM operation. Images of bacteria and poultry skin were obtained after staining with Pyronin‐Y. Data indicated that Salmonella cells were mostly located in the cervices and feather follicles. Salmonella in feather follicle floated freely in surrounding liquid even after the skin was thoroughly rinsed.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1996.tb01161.x