The effect of estrone and estradiol treatment on endometrial total protein, uteroferrin, and retinol-binding protein secretion during midpregnancy or midpseudopregnancy in swine

• Published in: Journal of animal science 1996-11, Vol.74 (11), p.2765-2772
• Main Authors: Vallet, J. L, Christenson, R. K
• Format: Article
• Language: English
• Subjects: acid phosphatase; Acid Phosphatase - analysis; Acid Phosphatase - metabolism; Animal reproduction; Animals; Biological and medical sciences; blood plasma; cerdas; controlled release; Endometrium - chemistry; Endometrium - enzymology; Endometrium - metabolism; Estradiol - analysis; Estradiol - blood; Estradiol - pharmacology; estrogenos; Estrogens; Estrone - analysis; Estrone - blood; Estrone - pharmacology; Female; fosfatasa acida; Fundamental and applied biological sciences. Psychology; gestacion; gestation; Hogs; Hormone metabolism and regulation; Isoenzymes; liberacion controlada; liberation controlee; Metalloproteins - metabolism; oestrogene; oestrogens; phosphatase acide; placenta; plasma sanguin; plasma sanguineo; Pregnancy; Pregnancy, Animal - metabolism; Pregnancy. Parturition. Lactation; proteinas; proteine; Proteins; Proteins - metabolism; pseudogestation; pseudopregnancy; Pseudopregnancy - metabolism; Pseudopregnancy - veterinary; Retinol-Binding Proteins - metabolism; Retinol-Binding Proteins, Plasma; secrecion; secretion; seudogestacion; sows; Swine - metabolism; Swine Diseases - metabolism; Tartrate-Resistant Acid Phosphatase; truie; utero; uterus; Vertebrates: reproduction
• ISSN: 0021-8812; 1525-3163; 0021-8812
• DOI: 10.2527/1996.74112765x

It has been hypothesized that conceptus estrogens influence endometrial protein secretion during pregnancy in swine. To test this hypothesis, the effect of estrone and estradiol treatment from d 30 to 60 of pregnancy or pseudopregnancy on endometrial protein secretion was investigated. Pregnant (P; n = 16) and pseudopregnant (PP) gilts (n = 18) received either sham treatment or estrone or estradiol implants (5 mg/d release rate; 60 d release) on d 30 of P or PP. Blood samples were collected on d 30, 40, 50, and 60 to measure estrone and estradiol. On d 60, gilts were hysterectomized. For P gilts, endometrium in apposition to one placenta from each uterine horn was collected. For PP gilts, each uterine horn was flushed with 40 mL of leucine-deficient minimal essential medium (MEM), and endometrial tissue was collected from each horn. Endometrial tissues were incubated in MEM in the presence of 50 microCi of [3H]leucine to examine protein secretion. Estrone and estradiol treatments increased both plasma and endometrial concentrations of estrone (P < 0.01 except endometrium for P gilts) and estradiol (P < 0.01, respectively). Endometrium from P gilts secreted more nondialyzable macromolecules (NDM), acid phosphatase activity (AP, a measure of uteroferrin), and retinol-binding protein (RBP) in culture than did endometrium from PP gilts. Estrone treatment increased (P < 0.01) endometrial NDM from P gilts but not that from PP gilts; estradiol had no effect. Both estrone and estradiol increased (P = 0.069) endometrial secretion of AP of PP but not of P gilts. Endometrial secretion of RBP was not affected by either estrone or estradiol treatment. Neither estrone nor estradiol affected total protein or AP and estrone treatment decreased (P < 0.05) RBP in uterine flushings from PP gilts. These data indicate that endometrium from P pigs secretes more protein than endometrium from PP pigs but neither estrone nor estradiol completely mimics the effect of pregnancy.