Cloning of Human RTEF-1, a Transcriptional Enhancer Factor-1-Related Gene Preferentially Expressed in Skeletal Muscle: Evidence for an Ancient Multigene Family

Transcriptional Enhancer Factor-1 (TEF-1) is a transcription factor required for cardiac muscle gene activation. Since ablation of TEF-1 does not abolish cardiac gene expression, we sought to identify a human gene related to TEF-1 (RTEF-1) that might also participate in cardiac gene regulation. A hu...

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Bibliographic Details
Published in:Genomics (San Diego, Calif.) Calif.), 1996-10, Vol.37 (1), p.68-76
Main Authors: Stewart, Alexandre F.R., Richard III, Charles W., Suzow, Joseph, Stephan, Dietrich, Weremowicz, Stanislawa, Morton, Cynthia C., Adra, Chaker N.
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Animals
Base Sequence
Biological and medical sciences
Chromosome Mapping
Chromosomes, Human, Pair 11
DNA, Complementary
DNA-Binding Proteins
Fundamental and applied biological sciences. Psychology
Genes. Genome
Humans
Molecular and cellular biology
Molecular genetics
Molecular Sequence Data
Multigene Family
Muscle Proteins - classification
Muscle Proteins - genetics
Muscle, Skeletal - metabolism
Phylogeny
RNA, Messenger - genetics
Trans-Activators - classification
Trans-Activators - genetics
Transcription Factors
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Summary:Transcriptional Enhancer Factor-1 (TEF-1) is a transcription factor required for cardiac muscle gene activation. Since ablation of TEF-1 does not abolish cardiac gene expression, we sought to identify a human gene related to TEF-1 (RTEF-1) that might also participate in cardiac gene regulation. A human heart cDNA library was screened to obtain a full-length RTEF-1 cDNA. Fluorescencein situhybridization assigned the RTEF-1 gene to chromosome 12p13.2–p13.3. In contrast, PCR screening of human/rodent cell hybrid panels identified TEF-1 on chromosome 11p15.2, between D11S1315 and D11S1334, extending a region of known synteny between human chromosomes 11 and 12 and arguing for an ancient divergence between these two closely related genes. Northern blot analysis revealed a striking similarity in the tissue distribution of RTEF-1 and TEF-1 mRNAs; skeletal muscle showed the highest abundance of both mRNAs, with lower levels detected in pancreas, placenta, and heart. Phylogenetic analysis of all known TEF-1-related proteins identified human RTEF-1 as one of four vertebrate members of this multigene family and further suggests that these genes diverged in the earliest metazoan ancestors.
ISSN:0888-7543
1089-8646
DOI:10.1006/geno.1996.0522