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HIV-1 gag-specific cytotoxic T lymphocytes defined with recombinant vaccinia virus and synthetic peptides
Current candidate vaccines fail to protect primates against challenge with human immunodeficiency virus (HIV) in the presence of antibody responses; this underlines the importance of studying cell-mediated immunity to HIV and identifying specific epitopes that stimulate cytotoxic T lymphocytes (CTL)...
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| Published in: | Nature (London) 1988-12, Vol.336 (6198), p.484-487 |
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| Main Authors: | , , , , , |
| Format: | Article |
| Language: | English |
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| cites | cdi_FETCH-LOGICAL-c365t-1f17cc91fde105f1479e7f49ecb358584a9f1f7a163fc5a552130037c7ab8b893 |
| container_end_page | 487 |
| container_issue | 6198 |
| container_start_page | 484 |
| container_title | Nature (London) |
| container_volume | 336 |
| creator | Nixon, Douglas F Townsend, Alain R. M Elvin, John G Rizza, Charles R Gallwey, John McMichael, Andrew J |
| description | Current candidate vaccines fail to protect primates against challenge with human immunodeficiency virus (HIV) in the presence of antibody responses; this underlines the importance of studying cell-mediated immunity to HIV and identifying specific epitopes that stimulate cytotoxic T lymphocytes (CTL). Using a recombinant vaccinia virus to express the gag protein of HIV-1 we found HLA class-I-restricted gag-specific CTL in thirteen out of fifteen healthy HIV seropositive patients. We then used short synthetic peptides in the lysis assay to screen for gag CTL epitopes. In one patient we have identified a peptide in p24 that is recognized by CTL in association with HLA-B27. This peptide, and further peptide sequences defined by these methods, could be incorporated in vaccines designed to induce cell-mediated immunity against HIV. |
| doi_str_mv | 10.1038/336484a0 |
| format | article |
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M ; Elvin, John G ; Rizza, Charles R ; Gallwey, John ; McMichael, Andrew J</creator><creatorcontrib>Nixon, Douglas F ; Townsend, Alain R. M ; Elvin, John G ; Rizza, Charles R ; Gallwey, John ; McMichael, Andrew J</creatorcontrib><description>Current candidate vaccines fail to protect primates against challenge with human immunodeficiency virus (HIV) in the presence of antibody responses; this underlines the importance of studying cell-mediated immunity to HIV and identifying specific epitopes that stimulate cytotoxic T lymphocytes (CTL). Using a recombinant vaccinia virus to express the gag protein of HIV-1 we found HLA class-I-restricted gag-specific CTL in thirteen out of fifteen healthy HIV seropositive patients. We then used short synthetic peptides in the lysis assay to screen for gag CTL epitopes. In one patient we have identified a peptide in p24 that is recognized by CTL in association with HLA-B27. This peptide, and further peptide sequences defined by these methods, could be incorporated in vaccines designed to induce cell-mediated immunity against HIV.</description><identifier>ISSN: 0028-0836</identifier><identifier>EISSN: 1476-4687</identifier><identifier>DOI: 10.1038/336484a0</identifier><identifier>PMID: 2461519</identifier><identifier>CODEN: NATUAS</identifier><language>eng</language><publisher>London: Nature Publishing</publisher><subject>Acquired immune deficiency syndrome ; Acquired Immunodeficiency Syndrome - immunology ; AIDS ; AIDS/HIV ; Antigens, Viral ; Biological and medical sciences ; DNA, Recombinant ; Epitopes - immunology ; Fundamental and applied biological sciences. Psychology ; Gene Products, gag ; HIV Antigens - immunology ; HIV Seropositivity ; HLA Antigens - immunology ; HLA-A Antigens - immunology ; HLA-A2 Antigen ; HLA-B Antigens - immunology ; HLA-B27 Antigen ; Human immunodeficiency virus 1 ; Humans ; Immunity, Cellular ; Lymphocytes ; Medical research ; Microbiology ; Peptide Fragments - immunology ; Peptides ; Primates ; Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains ; Retroviridae Proteins - immunology ; T-Lymphocytes, Cytotoxic - immunology ; Vaccines ; Vaccinia virus ; Vaccinia virus - immunology ; Virology</subject><ispartof>Nature (London), 1988-12, Vol.336 (6198), p.484-487</ispartof><rights>1990 INIST-CNRS</rights><rights>Copyright Macmillan Journals Ltd. 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M</creatorcontrib><creatorcontrib>Elvin, John G</creatorcontrib><creatorcontrib>Rizza, Charles R</creatorcontrib><creatorcontrib>Gallwey, John</creatorcontrib><creatorcontrib>McMichael, Andrew J</creatorcontrib><title>HIV-1 gag-specific cytotoxic T lymphocytes defined with recombinant vaccinia virus and synthetic peptides</title><title>Nature (London)</title><addtitle>Nature</addtitle><description>Current candidate vaccines fail to protect primates against challenge with human immunodeficiency virus (HIV) in the presence of antibody responses; this underlines the importance of studying cell-mediated immunity to HIV and identifying specific epitopes that stimulate cytotoxic T lymphocytes (CTL). Using a recombinant vaccinia virus to express the gag protein of HIV-1 we found HLA class-I-restricted gag-specific CTL in thirteen out of fifteen healthy HIV seropositive patients. We then used short synthetic peptides in the lysis assay to screen for gag CTL epitopes. In one patient we have identified a peptide in p24 that is recognized by CTL in association with HLA-B27. This peptide, and further peptide sequences defined by these methods, could be incorporated in vaccines designed to induce cell-mediated immunity against HIV.</description><subject>Acquired immune deficiency syndrome</subject><subject>Acquired Immunodeficiency Syndrome - immunology</subject><subject>AIDS</subject><subject>AIDS/HIV</subject><subject>Antigens, Viral</subject><subject>Biological and medical sciences</subject><subject>DNA, Recombinant</subject><subject>Epitopes - immunology</subject><subject>Fundamental and applied biological sciences. Psychology</subject><subject>Gene Products, gag</subject><subject>HIV Antigens - immunology</subject><subject>HIV Seropositivity</subject><subject>HLA Antigens - immunology</subject><subject>HLA-A Antigens - immunology</subject><subject>HLA-A2 Antigen</subject><subject>HLA-B Antigens - immunology</subject><subject>HLA-B27 Antigen</subject><subject>Human immunodeficiency virus 1</subject><subject>Humans</subject><subject>Immunity, Cellular</subject><subject>Lymphocytes</subject><subject>Medical research</subject><subject>Microbiology</subject><subject>Peptide Fragments - immunology</subject><subject>Peptides</subject><subject>Primates</subject><subject>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</subject><subject>Retroviridae Proteins - immunology</subject><subject>T-Lymphocytes, Cytotoxic - immunology</subject><subject>Vaccines</subject><subject>Vaccinia virus</subject><subject>Vaccinia virus - immunology</subject><subject>Virology</subject><issn>0028-0836</issn><issn>1476-4687</issn><fulltext>true</fulltext><rsrctype>article</rsrctype><creationdate>1988</creationdate><recordtype>article</recordtype><recordid>eNqFks1u1DAUhS0EKtMBiRcAWQihblJ8498sqwpopUpsWraR49gdV4kTbKdl3r6uZpgFm1ld655Pxzo-RugDkHMgVH2jVDDFNHmFVsCkqJhQ8jVaEVKriigq3qLTlB4IIRwkO0EnNRPAoVkhf3X9uwJ8r--rNFvjnTfYbPOUp7_ldIuH7ThvprKxCffW-WB7_OTzBkdrprHzQYeMH7UxPniNH31cEtahx2kb8sbm4jHbOfvepnfojdNDsu_3c43ufny_vbyqbn79vL68uKkMFTxX4EAa04DrLRDuSprGSscaazrKFS8hGwdOahDUGa45r4ESQqWRulOdaugafd35znH6s9iU29EnY4dBBzstqZWKc0HYcZDymqhGHgdrAFUrzo6CwAUoLmkBP_8HPkxLDOVZ2powRoHTF7ezHWTilFK0rp2jH3XctkDal9bbf60X9OPeb-lG2x_Afc1F_7LXdTJ6cFEH49MBE1xJVb7JGn3aYUHnJdqDfrjnGbFzvKY</recordid><startdate>19881201</startdate><enddate>19881201</enddate><creator>Nixon, Douglas F</creator><creator>Townsend, Alain R. 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M ; Elvin, John G ; Rizza, Charles R ; Gallwey, John ; McMichael, Andrew J</author></sort><facets><frbrtype>5</frbrtype><frbrgroupid>cdi_FETCH-LOGICAL-c365t-1f17cc91fde105f1479e7f49ecb358584a9f1f7a163fc5a552130037c7ab8b893</frbrgroupid><rsrctype>articles</rsrctype><prefilter>articles</prefilter><language>eng</language><creationdate>1988</creationdate><topic>Acquired immune deficiency syndrome</topic><topic>Acquired Immunodeficiency Syndrome - immunology</topic><topic>AIDS</topic><topic>AIDS/HIV</topic><topic>Antigens, Viral</topic><topic>Biological and medical sciences</topic><topic>DNA, Recombinant</topic><topic>Epitopes - immunology</topic><topic>Fundamental and applied biological sciences. Psychology</topic><topic>Gene Products, gag</topic><topic>HIV Antigens - immunology</topic><topic>HIV Seropositivity</topic><topic>HLA Antigens - immunology</topic><topic>HLA-A Antigens - immunology</topic><topic>HLA-A2 Antigen</topic><topic>HLA-B Antigens - immunology</topic><topic>HLA-B27 Antigen</topic><topic>Human immunodeficiency virus 1</topic><topic>Humans</topic><topic>Immunity, Cellular</topic><topic>Lymphocytes</topic><topic>Medical research</topic><topic>Microbiology</topic><topic>Peptide Fragments - immunology</topic><topic>Peptides</topic><topic>Primates</topic><topic>Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains</topic><topic>Retroviridae Proteins - immunology</topic><topic>T-Lymphocytes, Cytotoxic - immunology</topic><topic>Vaccines</topic><topic>Vaccinia virus</topic><topic>Vaccinia virus - immunology</topic><topic>Virology</topic><toplevel>peer_reviewed</toplevel><toplevel>online_resources</toplevel><creatorcontrib>Nixon, Douglas F</creatorcontrib><creatorcontrib>Townsend, Alain R. 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Using a recombinant vaccinia virus to express the gag protein of HIV-1 we found HLA class-I-restricted gag-specific CTL in thirteen out of fifteen healthy HIV seropositive patients. We then used short synthetic peptides in the lysis assay to screen for gag CTL epitopes. In one patient we have identified a peptide in p24 that is recognized by CTL in association with HLA-B27. This peptide, and further peptide sequences defined by these methods, could be incorporated in vaccines designed to induce cell-mediated immunity against HIV.</abstract><cop>London</cop><pub>Nature Publishing</pub><pmid>2461519</pmid><doi>10.1038/336484a0</doi><tpages>4</tpages><oa>free_for_read</oa></addata></record> |
| fulltext | fulltext |
| identifier | ISSN: 0028-0836 |
| ispartof | Nature (London), 1988-12, Vol.336 (6198), p.484-487 |
| issn | 0028-0836 1476-4687 |
| language | eng |
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| source | Nature |
| subjects | Acquired immune deficiency syndrome Acquired Immunodeficiency Syndrome - immunology AIDS AIDS/HIV Antigens, Viral Biological and medical sciences DNA, Recombinant Epitopes - immunology Fundamental and applied biological sciences. Psychology Gene Products, gag HIV Antigens - immunology HIV Seropositivity HLA Antigens - immunology HLA-A Antigens - immunology HLA-A2 Antigen HLA-B Antigens - immunology HLA-B27 Antigen Human immunodeficiency virus 1 Humans Immunity, Cellular Lymphocytes Medical research Microbiology Peptide Fragments - immunology Peptides Primates Replicative cycle, interference, host-virus relations, pathogenicity, miscellaneous strains Retroviridae Proteins - immunology T-Lymphocytes, Cytotoxic - immunology Vaccines Vaccinia virus Vaccinia virus - immunology Virology |
| title | HIV-1 gag-specific cytotoxic T lymphocytes defined with recombinant vaccinia virus and synthetic peptides |
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