Localization of 28 kDa calbindin in human odontoblasts

The presence of 28 kDa calbindin in human odontoblasts was studied by use of specific antibodies raised against chick duodenal 28 kDa calbindin, in immunofluorescence, immuno-peroxidase, and electron-microscopic labelling experiments. The calbindin-like protein was detected mainly in the cytoplasm o...

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Bibliographic Details
Published in:Cell and tissue research 1988-11, Vol.254 (2), p.341-346
Main Authors: Magloire, H, Joffre, A, Azerad, J, Lawson, D E
Format: Article
Language:English
Subjects:
Animals
Calbindins
Cell Nucleus - analysis
Cell Nucleus - ultrastructure
Cytoplasm - analysis
Cytoplasm - ultrastructure
Humans
Immunohistochemistry
Mice
Mice, Inbred Strains
Microscopy, Electron
Molar - analysis
Molar - ultrastructure
Odontoblasts - analysis
Odontoblasts - ultrastructure
S100 Calcium Binding Protein G - analysis
Tooth - analysis
Tooth - ultrastructure
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Summary:The presence of 28 kDa calbindin in human odontoblasts was studied by use of specific antibodies raised against chick duodenal 28 kDa calbindin, in immunofluorescence, immuno-peroxidase, and electron-microscopic labelling experiments. The calbindin-like protein was detected mainly in the cytoplasm of odontoblast cell bodies, in their processes and occasionally in their nuclei. Correspondingly, at the ultrastructural level, immunoreactive material was associated with the cytosol, microfilaments and cilia. These findings suggest that human odontoblasts express a 28 kDa vitamin D-dependent calcium-binding protein, unlike those of rats and mice in which ameloblasts are the only cells immunoreactive for the protein.
ISSN:0302-766X
1432-0878
DOI:10.1007/BF00225806