Isolation and characterization of the Haemophilus influenzae uvrA gene

The uvrA gene of Haemophilus influenzae (Hi) was cloned and sequenced. Analysis of the deduced amino acid sequence revealed 81% identity and 90% similarity with the Escherichia coli UvrA protein. Consistent with a role of Hi uvrA in DNA repair, a Hi uvrA mutant exhibited increased sensitivity of UV...

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Bibliographic Details
Published in:Gene 1996-10, Vol.177 (1), p.23-28
Main Authors: de la Morena, Maria L., Hendrixson, David R., St. Geme, Joseph W.
Format: Article
Language:English
Subjects:
Adenosine Triphosphatases - genetics
Amino Acid Sequence
Bacterial Proteins - genetics
Base Sequence
Cloning, Molecular
DNA damage
DNA Repair
DNA, Bacterial
DNA-Binding Proteins - genetics
Escherichia coli Proteins
Excinuclease
Genes, Bacterial
Genetic Complementation Test
Haemophilus influenzae
Haemophilus influenzae - enzymology
Haemophilus influenzae - genetics
Haemophilus influenzae - radiation effects
Molecular Sequence Data
Nucleotide excision
Sequence Homology, Amino Acid
Ultraviolet Rays
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Summary:The uvrA gene of Haemophilus influenzae (Hi) was cloned and sequenced. Analysis of the deduced amino acid sequence revealed 81% identity and 90% similarity with the Escherichia coli UvrA protein. Consistent with a role of Hi uvrA in DNA repair, a Hi uvrA mutant exhibited increased sensitivity of UV irradiation. Furthermore, Hi uvrA was able to complement a mutation in the E. coli uvrA locus.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(96)00264-8