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Cloning and expression of the chicken protein tyrosine phosphatase SH-PTP2

SH-PTP2 is a protein tyrosine phosphatase which contains two src homology 2 (SH2) domains. A partial cDNA clone encoding chicken SH-PTP2 was generated by RT-PCR and used as a probe to screen several chicken cDNA libraries. Two overlapping cDNA clones were identified and the nucleotide sequence of ch...

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Bibliographic Details
Published in:Gene 1996-10, Vol.177 (1), p.93-97
Main Authors: Park, Chong Yon, Lamontagne, Kenneth R., Tonks, Nicholas K., Hayman, Michael J.
Format: Article
Language:English
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Summary:SH-PTP2 is a protein tyrosine phosphatase which contains two src homology 2 (SH2) domains. A partial cDNA clone encoding chicken SH-PTP2 was generated by RT-PCR and used as a probe to screen several chicken cDNA libraries. Two overlapping cDNA clones were identified and the nucleotide sequence of chicken SH-PTP2 containing the entire protein-coding region was determined. The deduced amino acid sequence shares 98% and 94% identity, respectively with the corresponding human and Xenopus proteins. Northern and Western blot analyses show that chicken SH-PTP2 is expressed ubiquitously like those of mammals and Xenopus. This suggests that chicken SH-PTP2 may have analogous biological roles to those of mammals.
ISSN:0378-1119
1879-0038
DOI:10.1016/0378-1119(96)00278-8