An in-vitro evaluation of the activity of povidone-iodine against nosocomial bacterial strains

• Published in: The Journal of hospital infection 1996-11, Vol.34 (3), p.217-222
• Main Authors: Traoré, O., Fayard, S.Fournet, Laveran, H.
• Format: Article
• Language: English
• Subjects: Anti-Infective Agents, Local - pharmacology; antibiotic multi-resistant bacteria; Antibiotics. Antiinfectious agents. Antiparasitic agents; Antiseptics; Bacterial Infections - prevention & control; bactericidal activity; Biological and medical sciences; Cross Infection - prevention & control; Drug Resistance, Microbial; Humans; In Vitro Techniques; Infection Control - methods; Medical sciences; Microbial Sensitivity Tests - methods; micromethod; Pharmacology. Drug treatments; Povidone-Iodine - pharmacology; Povidone—iodine
• ISSN: 0195-6701; 1532-2939
• DOI: 10.1016/S0195-6701(96)90069-9

Two povidone—iodine (PVP-I) preparations, one, an antiseptic handwash and one, a skin disinfectant, were tested against 504 bacterial strains isolated from nosocomial infections in 12 French hospitals. In-vitro bactericidal activity was determined by a micromethod, using specific interfering substances over a range of dilutions, after 1, 3 and 5 min exposure times. A 5 log10 reduction of the challenge inoculum was considered as the criterion of efficacy. Any resistant strains were tested with the French Standard (T72300). When the micromethod was carried out at 20 °C, 10·7% (54/504) of the strains were resistant to the PVP-I skin disinfectant (dilution 1:10) and 1·6% (8/504) were resistant to the handwashing formulations (dilution 1:3) after 1 min exposure. By increasing the temperature to 32 °C, the resistance rate to the skin disinfectant fell to 1·9% (10/504). All of the 18 strains found resistant with the micromethod were sensitive using the French standard.