Recombinant Vaccinia/Venezuelan Equine Encephalitis (VEE) Virus Expresses VEE Structural Proteins

• Published in: Journal of general virology 1988-12, Vol.69 (12), p.3005-3013
• Main Authors: Kinney, Richard M, Esposito, Joseph J, Johnson, Barbara J. B, Roehrig, John T, Mathews, James H, Barrett, Alan D. T, Trent, Dennis W
• Format: Article
• Language: English
• Subjects: Antibodies, Monoclonal; Base Sequence; Biological and medical sciences; Cloning, Molecular; DNA; Encephalitis Virus, Venezuelan Equine - genetics; Encephalitis Virus, Venezuelan Equine - metabolism; Epitopes - genetics; Fluorescent Antibody Technique; Fundamental and applied biological sciences. Psychology; Genetics; Immunoblotting; Microbiology; Plasmids; Recombinant Fusion Proteins - biosynthesis; Recombinant Fusion Proteins - genetics; Recombinant Proteins - biosynthesis; RNA, Viral; vaccinia virus; Vaccinia virus - genetics; Vaccinia virus - metabolism; Venezuelan equine encephalitis virus; Viral Envelope Proteins - biosynthesis; Viral Envelope Proteins - genetics; Viral Proteins - biosynthesis; Viral Proteins - genetics; Viral Structural Proteins; Virology
• ISSN: 0022-1317; 1465-2099
• DOI: 10.1099/0022-1317-69-12-3005

1 Division of Vector-Borne Viral Diseases, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, P.O. Box 2087, Fort Collins, Colorado 80522, 2 Division of Viral Diseases, Center for Infectious Diseases, Centers for Disease Control, Public Health Service, U.S. Department of Health and Human Services, 1600 Clifton Road, Atlanta, Georgia 30333, U.S.A. and 3 Department of Microbiology, University of Surrey, Guildford GU2 5XH, Surrey, U.K. cDNA molecules encoding the structural proteins of the virulent Trinidad donkey and the TC-83 vaccine strains of Venezuelan equine encephalitis (VEE) virus were inserted under control of the vaccinia virus 7.5K promoter into the thymidine kinase gene of vaccinia virus. Synthesis of the capsid protein and glycoproteins E2 and E1 of VEE virus was demonstrated by immunoblotting of lysates of CV-1 cells infected with recombinant vaccinia/VEE viruses. VEE glycoproteins were detected in recombinant virus-infected cells by fluorescent antibody (FA) analysis performed with a panel of VEE-specific monoclonal antibodies. Seven E2-specific epitopes and two of four E1-specific epitopes were demonstrated by FA. Keywords: vaccinia virus, VEE virus, recombinant virus Received 11 July 1988; accepted 18 August 1988.