Investigation of an Apparent Cluster of Klebsiella pneumoniae Bacteremias Using Random Amplified Polymorphic DNA Analysis

A cluster of bacteremia episodes with Klebsiella pneumoniae was noted in patients in a hematology-oncology ward during a 3-week period. Random amplified polymorphic DNA (RAPD) analysis, a novel technique for generating chromosomal fingerprints from bacterial isolates, was used as an aid to the epide...

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Bibliographic Details
Published in:Infection control and hospital epidemiology 1996-11, Vol.17 (11), p.743-745
Main Authors: Hurley, James C., Russell, Edward G., Harrington, Glenys, Spicer, W. John
Format: Article
Language:English
Subjects:
Bacteremia
Bacteremia - microbiology
Bacterial diseases
Bacterial sepsis
Biochemistry
Biological and medical sciences
Blood
Concise Communications
Cross Infection - microbiology
DNA
Genetic polymorphism
Hematology
Hospital units
Human bacterial diseases
Humans
Infection Control
Infectious diseases
Klebsiella
Klebsiella Infections - microbiology
Klebsiella pneumoniae
Klebsiella pneumoniae - classification
Klebsiella pneumoniae - genetics
Medical sciences
Nursing
Polymerase chain reaction
Random Amplified Polymorphic DNA Technique
Serotyping
Space-Time Clustering
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Summary:A cluster of bacteremia episodes with Klebsiella pneumoniae was noted in patients in a hematology-oncology ward during a 3-week period. Random amplified polymorphic DNA (RAPD) analysis, a novel technique for generating chromosomal fingerprints from bacterial isolates, was used as an aid to the epidemiological investigation of this cluster. For each of the two patients from whom multiple isolates had been obtained, identical RAPD patterns were observed in the serial isolates, even for a patient where the isolates had different biotypes. Isolates from different patients gave distinct patterns. Random amplified polymorphic DNA was found to be a useful typing technique for this cluster of K pneumonia bacteremias.
ISSN:0899-823X
1559-6834
DOI:10.1086/647221