Transformation of Streptococcus bovis protoplasts by plasmid DNA

M. MAREKOVÁ, V. KMET' AND P. JAVORSKÝ. 1996. The transformation and subsequent regeneration of ruminal strain Streptococcus bovis AO24/85 protoplasts by plasmid DNA was studied. The best stabilizer for regeneration of protoplasted cells was 5% sucrose in the regeneration medium and in the agar...

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Bibliographic Details
Published in:Letters in applied microbiology 1996-02, Vol.22 (2), p.159-161
Main Authors: Mareková, M., Kmet, V., Javorský, P.
Format: Article
Language:English
Subjects:
Animals
Biological and medical sciences
Biotechnology
Cattle
DNA, Bacterial - genetics
DNA, Bacterial - isolation & purification
Fundamental and applied biological sciences. Psychology
Genetic engineering
Genetic technics
Genetic Techniques
Methods. Procedures. Technologies
Miscellaneous
Plasmids - genetics
Polyethylene Glycols
Protoplasts
Rumen - microbiology
Streptococcus bovis
Streptococcus bovis - genetics
Sucrose
Synthetic digonucleotides and genes. Sequencing
Transformation, Genetic
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Summary:M. MAREKOVÁ, V. KMET' AND P. JAVORSKÝ. 1996. The transformation and subsequent regeneration of ruminal strain Streptococcus bovis AO24/85 protoplasts by plasmid DNA was studied. The best stabilizer for regeneration of protoplasted cells was 5% sucrose in the regeneration medium and in the agar plates. Optimal concentration of polyethylene glycol 6000 in the transformation medium was 25% for both plasmids tested. Addition of Ca2+ and Mg2+ ions (2.5 mmol l‐1) to the transformation medium increased the proportion of regenerated cells. Transformation frequencies were 3 times 103 transformants per μg of pNZ12 and 2.4 times 102 per μg of pJK108, respectively.
ISSN:0266-8254
1472-765X
DOI:10.1111/j.1472-765X.1996.tb01132.x