Period Protein Is Necessary for Circadian Control of Egg Hatching Behavior in the Silkmoth Antheraea pernyi

We examined the molecular basis of the circadian control of egg hatching behavior in the silkmoth Antheraea pernyi. Egg hatching is rhythmically gated, persists under constant darkness, and can be entrained by light by midembryogenesis. The time of appearance of photic entrainment by the silkmoth em...

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Bibliographic Details
Published in:Neuron (Cambridge, Mass.) Mass.), 1996-11, Vol.17 (5), p.901-909
Main Authors: Sauman, Ivo, Tsai, Tony, Roca, Alfred L, Reppert, Steven M
Format: Article
Language:English
Subjects:
Animals
Antheraea pernyi
Antherarea pernyi
Antibody Specificity
Circadian Rhythm - physiology
Drosophila Proteins
Embryo, Nonmammalian - physiology
Female
Immunohistochemistry
Larva - physiology
Male
Microinjections
Moths - physiology
Nuclear Proteins - analysis
Nuclear Proteins - immunology
Nuclear Proteins - physiology
Oligonucleotides - pharmacology
PER proteins
Period Circadian Proteins
Proteins - analysis
Proteins - immunology
Proteins - physiology
Saturniidae
TIM proteins
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Summary:We examined the molecular basis of the circadian control of egg hatching behavior in the silkmoth Antheraea pernyi. Egg hatching is rhythmically gated, persists under constant darkness, and can be entrained by light by midembryogenesis. The time of appearance of photic entrainment by the silkmoth embryo coincides with the appearance of Period (PER) and Timeless (TIM) proteins in eight cells in embryonic brain. Although daily rhythms in PER and/or TIM immunoreactivity in embryonic brain were not detected, a robust circadian oscillation of PER immunoreactivity is present in the nuclei of midgut epithelium. per antisense oligodeoxynucleotide treatment of pharate larvae on the day before hatching consistently abolishes the circadian gate of egg hatching behavior. per antisense treatment also causes a dramatic decrease in PER immunoreactivity in newly hatched larvae. The results provide direct evidence that PER is a necessary element of a circadian system in the silkmoth.
ISSN:0896-6273
1097-4199
DOI:10.1016/S0896-6273(00)80221-4