Use of AFLP Markers for Gene Mapping and QTL Detection in the Rat

• Published in: Genomics (San Diego, Calif.) Calif.), 1996-11, Vol.37 (3), p.289-294
• Main Authors: Otsen, Myrthe, den Bieman, Maria, Kuiper, Martin T.R., Pravenec, Michal, Kren, Vladimir, Kurtz, Theodore W., Jacob, Howard J., Lankhorst, Ægidius, van Zutphen, Bert F.M.
• Format: Article
• Language: English
• Subjects: Animals; Biological and medical sciences; Blood Pressure - genetics; Chromosome Mapping - methods; Classical genetics, quantitative genetics, hybrids; Crosses, Genetic; Female; Fundamental and applied biological sciences. Psychology; Genetic Markers; Genetics of eukaryotes. Biological and molecular evolution; Male; Phenotype; Polymerase Chain Reaction - methods; Polymorphism, Restriction Fragment Length; Rats - genetics; Rats, Inbred ACI; Rats, Inbred BN; Vertebrata
• ISSN: 0888-7543; 1089-8646
• DOI: 10.1006/geno.1996.0562

The AFLP technique is a new DNA marker technology based on the selective amplification of restriction fragments. Multiple polymorphic markers are simultaneously produced and can be tested in one PCR. No prior information on genomic DNA sequences is needed. In the current study, we contribute 18 AFLP markers to the linkage map of the rat. Seven AFLP markers were assigned to specific chromosomes by analysis of a (BN × ACI)F1 × ACI backcross progeny. Another 11 AFLP markers were mapped by using a panel of the H × B/B × H recombinant inbred (RI) strains. Genotypes of these AFLP markers were also tested for correlations with some blood pressure phenotypes in the RI strains. Suggestive correlation was found between the mean arterial pressure and two closely linked AFLP markers located on chromosome 20. The current study illustrates the value of AFLP markers for the construction of linkage maps and the detection of quantitative trait loci.